Mammalian Na+/H+ exchanger gene family: structure and function studies.
Mammalian Na+/H+ exchanger gene family: structure and function studies.
复制标题
哺乳动物 Na /H 交换基因家族：结构和功能研究。
DOI：
10.1152/ajpgi.1995.269.1.g1
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Donowitz,M
中科院分区：
文献类型：
--
作者：
Yun,CH;Tse,CM;Nath,SK;Levine,SA;Brant,SR;Donowitz,M
Na+/H+交换剂是以一比一的化学计量将细胞外Na+交换为细胞内H+的完整质膜蛋白。它们可被利尿剂阿米洛利替代，并具有多种细胞功能，包括细胞内pH稳态、细胞体积控制和上皮细胞中的电中性NaCl吸收。通过最近克隆的四种哺乳动物Na+/H+交换剂NHE 1、NHE 2、NHE 3和NHE 4，证明存在多种形式的交换剂。所有这些克隆的Na+/H+交换器具有10-12个推定的跨膜螺旋和长的胞质羧基结构域。尽管结构相似，这些Na+/H+交换异构体不同的组织分布，动力学特性，和对外部刺激的反应。本文综述了Na+/H+交换基因家族的分子鉴定、功能特点以及Na+/H+交换在分子和细胞水平上的短期调控等方面的最新进展。
Na+/H+ exchangers are integral plasma membrane proteins that exchange extracellular Na+ for intracellular H+ with a stoichiometry of one for one. They are inhibitable by the diuretic amiloride and have multiple cellular functions, including intracellular pH homeostasis, cell volume control, and electroneutral NaCl absorption in epithelia. The presence of multiple forms of the exchangers was demonstrated by the recent cloning of four mammalian Na+/H+ exchangers, NHE1, NHE2, NHE3, and NHE4. All of these cloned Na+/H+ exchangers have 10-12 putative transmembrane helixes and a long cytoplasmic carboxyl domain. Despite the structural similarity, these Na+/H+ exchanger isoforms differ in their tissue distribution, kinetic characteristics, and response to external stimuli. The present review deals with the recent developments in the molecular identification of the Na+/H+ exchanger gene family, the functional characteristics, and the short-term regulation of Na+/H+ exchange at molecular and cellular levels.