Cytotoxic clinical isolates of Pseudomonas aeruginosa identified during the Steroids for Corneal Ulcers Trial show elevated resistance to fluoroquinolones.
Cytotoxic clinical isolates of Pseudomonas aeruginosa identified during the Steroids for Corneal Ulcers Trial show elevated resistance to fluoroquinolones.
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DOI：
10.1186/1471-2415-14-54
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发表时间：
2014-04-24
影响因子：
2
通讯作者：
Fleiszig SM
中科院分区：
文献类型：
--
作者：
Borkar DS;Acharya NR;Leong C;Lalitha P;Srinivasan M;Oldenburg CE;Cevallos V;Lietman TM;Evans DJ;Fleiszig SM
确定类固醇治疗角膜溃疡试验（SCUT）期间从微生物性角膜炎中分离的铜绿假单胞菌菌株和两种实验室菌株PA 103和PAO 1的3型分泌基因型与氟喹诺酮耐药性之间的关系。从SCUT中确认的铜绿假单胞菌分离株分为exoU（+）或exoU（−）。exoU（+）菌株含有编码ExoU的基因，ExoU是一种通过3型分泌系统递送到宿主细胞中的强大磷脂酶毒素。然后使用纸片扩散法评估分离株对氟喹诺酮、头孢菌素和氨基糖苷类抗生素的敏感性。Etest法测定氟喹诺酮类药物的最低抑菌浓度。还测试了添加或缺失exoU基因的实验室分离株。与exoU（−）分离株相比，exoU（+）菌株对环丙沙星（p = 0.001）、加替沙星（p = 0.003）和氧氟沙星（p = 0.002）耐药的比例显著较高。exoU（+）或exoU（-）阴性分离株对除庆大霉素外的其他抗生素的敏感性无显著差异。涉及耐药exoU（+）菌株的感染倾向于更差的临床结果。实验室分离株exoU的缺失或获得不影响氟喹诺酮类药物的敏感性。从SCUT中分离的铜绿假单胞菌对氟喹诺酮的敏感性与先前的研究一致，表明涉及exoU编码（细胞毒性）菌株的耐药性升高，并表明涉及耐药菌株的感染的临床结局更差。检测铜绿假单胞菌临床分离株中exoU的表达水平可能有助于指导微生物性角膜炎患者的临床治疗。
To determine the relationship between type three secretion genotype and fluoroquinolone resistance for P. aeruginosa strains isolated from microbial keratitis during the Steroids for Corneal Ulcers Trial (SCUT) and for two laboratory strains, PA103 and PAO1. Confirmed P. aeruginosa isolates from the SCUT were divided into exoU(+) or exoU(−). The exoU(+) strains contained the gene encoding ExoU, a powerful phospholipase toxin delivered into host cells by the type three secretion system. Isolates were then assessed for susceptibility to fluoroquinolone, cephalosporin, and aminoglycoside antibiotics using disk diffusion assays. Etest was used to determine the MIC of moxifloxacin and other fluoroquinolones. Laboratory isolates in which the exoU gene was added or deleted were also tested. A significantly higher proportion of exoU(+) strains were resistant to ciprofloxacin (p = 0.001), gatifloxacin (p = 0.003), and ofloxacin (p = 0.002) compared to exoU(−) isolates. There was no significant difference between exoU(+) or exoU(−) negative isolates with respect to susceptibility to other antibiotics except gentamicin. Infections involving resistant exoU(+) strains trended towards worse clinical outcome. Deletion or acquisition of exoU in laboratory isolates did not affect fluoroquinolone susceptibility. Fluoroquinolone susceptibility of P. aeruginosa isolated from the SCUT is consistent with previous studies showing elevated resistance involving exoU encoding (cytotoxic) strains, and suggest worse clinical outcome from infections involving resistant isolates. Determination of exoU expression in clinical isolates of P. aeruginosa may be helpful in directing clinical management of patients with microbial keratitis.
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影响因子：
8.1
作者：
Borkar, Durga S.;Fleiszig, Suzanne M. J.;Leong, Chelsia;Lalitha, Prajna;Srinivasan, Muthiah;Ghanekar, Avanti A.;Tam, Connie;Li, Wing Y.;Zegans, Michael E.;McLeod, Stephen D.;Lietman, Thomas M.;Acharya, Nisha R.
通讯作者：
Acharya, Nisha R.
影响因子：
2.8
作者：
Green, Matthew;Apel, Andrew;Stapleton, Fiona
通讯作者：
Stapleton, Fiona
影响因子：
4.4
作者：
Lee, EJ;Evans, DJ;Fleiszig, SMJ
通讯作者：
Fleiszig, SMJ
影响因子：
4.2
作者：
Chen, Aiyin;Prajna, Lalitha;Acharya, Nisha R.
通讯作者：
Acharya, Nisha R.
影响因子：
3.6
作者：
FinckBarbancon, V;Goranson, J;Frank, DW
通讯作者：
Frank, DW