Myeloma with multiple rearranged immunoglobulin kappa genes: only one kappa gene codes for kappa chains.
Myeloma with multiple rearranged immunoglobulin kappa genes: only one kappa gene codes for kappa chains.
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具有多个重排免疫球蛋白 kappa 基因的骨髓瘤：只有一个 kappa 基因编码 kappa 链。
DOI：
10.1093/nar/8.20.4681
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发表时间：
1980
影响因子：
14.9
通讯作者：
Wilson,R
中科院分区：
文献类型：
--
作者：
Storb,U;Arp,B;Wilson,R
在许多骨髓瘤中，不止一个kappa基因重排（2-5）。我们在这里报告的研究结果，以确定是否所有的重排基因的表达。发现在骨髓瘤NS-1中存在三种不同的重排kappa基因。在NS-1亚系和几种小鼠脾细胞与NS-1融合产生的杂交瘤中，发现NS-1 kappa链的产生与三种kappa基因中的一种存在相关。这个“表达”基因的缺失消除了NS-1 kappa链的合成，而另外两个重排kappa基因中的一个的缺失则没有。据推测，kappa基因的等位基因排斥（20）通常是通过一个kappa基因的功能性重排来实现的；其他重排相对频繁，至少在骨髓瘤中，但它们大多是非功能性的，因此不会产生混乱的抗体分子。
In many myelomas more than one kappa gene is rearranged (2–5). We are reporting here the results of studies undertaken to determine whether all the rearranged genes are expressed. It was found that in the myeloma NS-1 three different rearranged kappa genes exist. In a subline of NS-1 and several hybridomas produced by fusion of mouse spleen cells with NS-1 it was found that production of NS-1 kappa chains was correlated with the presence of one of the three kappa genes. Loss of this “expressed” gene eliminated the synthesis of the NS-1 kappa chains, loss of one of the other two rearranged kappa genes did not. It is hypothesized, that allelic exclusion (20) of kappa genes generally operates by the functional rearrangement of one kappa gene; other rearrangements are relatively frequent, at least in myelomas, but mostly they are nonfunctional and thus scrambled antibody molecules do not arise.