Activation of adenovirus promoters by the adenovirus E1A protein in cell-free extracts.
Activation of adenovirus promoters by the adenovirus E1A protein in cell-free extracts.
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无细胞提取物中腺病毒 E1A 蛋白激活腺病毒启动子。
DOI：
10.1126/science.2956686
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Harter,ML
中科院分区：
文献类型：
--
作者：
Spangler,R;Bruner,M;Dalie,B;Harter,ML
腺病毒E1 A基因的主要产物是一种足以控制宿主细胞增殖和使原代啮齿动物细胞永生化的蛋白质。该蛋白诱导这些细胞效应的机制知之甚少，但可能与其调节许多病毒和细胞基因的RNA转录的能力有关。本文通过检测E1 A蛋白在两种体外转录系统中对腺病毒特异性启动子的作用，研究了E1 A蛋白的转录激活（反式激活）机制。其中一个系统由组成型表达E1 A的转化细胞的提取物组成，另一个系统由补充有从大肠杆菌纯化的质粒编码的E1 A蛋白的HeLa细胞的提取物组成。结果表明，E1 A蛋白特异性地刺激腺病毒启动子的转录;因此，细胞转录因子的诱导对于解释E1 A对转录的刺激是不必要的。
The primary product of the adenovirus E1A gene is a protein that is sufficient for controlling host-cell proliferation and immortalizing primary rodent cells. The mechanism by which the protein induces these cellular effects is poorly understood, but might be linked to its ability to regulate RNA transcription from a number of viral and cellular genes. The mechanism of E1A's transcriptional-activation (trans-activation) was studied here by monitoring the protein's effect on specific adenovirus promoters in two types of transcriptional systems in vitro. One of these systems consisted of extracts from transformed cells constitutively expressing E1A, and the other consisted of extracts of HeLa cells supplemented with a plasmid-encoded E1A protein purified fromEscherichia coli. The results show that the E1A protein specifically stimulates transcription from adenovirus promoters; thus, the induction of cellular transcription factors is not necessary to explain the stimulation of transcription by E1A.