Assessment of liver function-related mRNA expression and fluorescence imaging in outflow-obstructed regions in rats
Assessment of liver function-related mRNA expression and fluorescence imaging in outflow-obstructed regions in rats
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大鼠流出受阻区肝功能相关 mRNA 表达及荧光成像评估
DOI：
10.1007/s00595-022-02588-3
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发表时间：
2022
期刊：
影响因子：
2.5
通讯作者：
Hasegawa Kiyoshi
中科院分区：
文献类型：
--
作者：
Ito Daisuke;Kawaguchi Yoshikuni;Inagaki Yoshinori;Ito Kyoji;Mihara Yuichiro;Kaneko Junichi;Tanaka Mariko;Fukayama Masashi;Kokudo Norihiro;Hasegawa Kiyoshi
目的肝血流流出道阻塞区的肝功能受损，但其程度尚未定量评估。因此，我们评估了吲哚菁绿色（ICG）到肝细胞的摄取和mRNA的表达与肝功能在流出道阻塞区域使用大鼠models.MethodsA共20只大鼠结扎右肝正中静脉，诱导流出道阻塞进行了研究。每组5只大鼠按再次剖腹时间和ICG荧光强度（FI）值分组。流出道梗阻组中白蛋白、细胞色素P450（Cyp）1a 2、Cyp 3a 1、Cyp 7a 1和γ-谷氨酰半胱氨酸合成酶的mRNA表达结果显微荧光成像显示，在12 h，24 h，肝静脉结扎后3天。mRNAOut-Ob/mRNANon比值在流出道梗阻后12 h下降至约30%，7 d时上升至约70-80%。我们的研究结果可能有助于估计术后功能性残肝体积考虑在流出道阻塞区域的肝功能下降。
PurposeThe liver function in outflow-obstructed regions is reportedly impaired; however, the functional decrease has not been quantitatively assessed. We therefore evaluated the uptake of indocyanine green (ICG) into hepatocytes and the mRNA expression associated with the liver function in outflow-obstructed regions using rat models.MethodsA total of 20 rats with the ligation of the right median hepatic vein to induce outflow obstruction were studied. Five rats each were grouped by the time of re-laparotomy, and the fluorescence intensity (FI) values of ICG. The mRNA expression, including that ofAlbumin,Cytochrome P450(Cyp)1a2,Cyp3a1, Cyp7a1, andGamma-glutamylcysteine synthetase, in outflow-obstructed (mRNAOut-Ob) and non-outflow-obstructed (mRNANon) regions was assessed.ResultsMicroscopic fluorescence imaging showed that the FI values were significantly lower in outflow-obstructed regions than in non-outflow-obstructed regions at 12 h, 24 h, and 3 days after ligation of the hepatic vein. The mRNAOut-Ob/mRNANonratios decreased to approximately 30% at 12 h after the outflow obstruction and increased to approximately 70–80% at 7 days.ConclusionsThe liver function in outflow-obstructed regions was impaired in terms of the uptake of ICG and the mRNA expression. Our findings may help estimate the postoperative functional remnant liver volume by considering the decrease in the liver function in outflow-obstructed regions.
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发表时间：
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