Diversity of microorganisms in decaying maize stalks revealed by a molecular method.
Diversity of microorganisms in decaying maize stalks revealed by a molecular method.
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发表时间：
2007-08
影响因子：
3
通讯作者：
Ming-Xia Yang;Han-Bo Zhang
中科院分区：
文献类型：
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作者：
Ming-Xia Yang;Han-Bo Zhang
通过构建和分析rRNA基因克隆文库，研究了腐熟玉米秸秆中微生物的多样性。从82个细菌克隆中共获得47个OTU，包括变形杆菌(64.6%)、放线杆菌(30.5%)、类杆菌(2.4%)和细菌(2.4%)。大多数蛋白细菌克隆为根瘤菌、假单胞菌和营养狭窄性单胞菌。84%的放线菌与微杆菌有关。从124个真菌克隆中只鉴定出14个OTUS，包括子囊菌(88%)和担子菌(12%)。子囊菌门中60%为真青霉和拟青霉属，担子菌门均与黑曲霉相近。
Microbial diversity in decaying maize stalk was characterized by constructing and analyzing rRNA gene clone library. Total 47 OTUs were obtained from 82 bacterial clones, including Proteobacteria (64.6%), Actinobacteria (30.5%), Bacteroidetes (2.4%) and Firmicutes (2.4%). Most proteobacterial clones were members of Rhizobium, Pseudomonas and Stenotrophomonas. Eighty-four percent of Actinobacteria was related to Microbacterium. Only 14 OTUs were identified from 124 fungal clones, including Ascomycota (88%) and Basidiomycota (12%). Sixty percent of Ascomycota were members of Eupenicillium and Paecilomyces but all Basidiomycota were close to Kurtzmanomyces nectairei.