MAINTENANCE OF 3T3-L1 CELLS IN CULTURE MEDIA CONTAINING SATURATED FATTY-ACIDS DECREASES INSULIN BINDING AND INSULIN ACTION
MAINTENANCE OF 3T3-L1 CELLS IN CULTURE MEDIA CONTAINING SATURATED FATTY-ACIDS DECREASES INSULIN BINDING AND INSULIN ACTION
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DOI：
10.1016/0006-291x(81)91682-x
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
KAHN, CR
中科院分区：
文献类型：
--
作者：
GRUNFELD, C;BAIRD, KL;KAHN, CR
为了改变细胞和膜脂的组成，分化的3T3-L1脂肪细胞被保存在含有透析的胎牛血清和添加各种脂肪酸的培养液中。暴露于饱和脂肪酸的细胞表现出高达80%的胰岛素结合减少，胰岛素刺激2-脱氧葡萄糖摄取；暴露于相同链长的单不饱和脂肪酸的细胞表现出较小的变化。胰岛素结合量的减少似乎是由于受体数量和亲和力的变化。脱氧葡萄糖对胰岛素的最大刺激作用降低，对胰岛素的敏感性变化不大。受试脂肪酸对胰岛素结合的抑制作用与胰岛素作用之间有很好的相关性。细胞脂质的修饰显然会对胰岛素结合和胰岛素作用产生深远的影响。
To modify cellular and membrane lipid composition, differentiated 3T3-L1 adipose cells were maintained in media containing dialyzed fetal calf serum supplemented with a variety of fatty acids. Cells exposed to saturated fatty acids exhibited up to an 80% decrease in insulin binding and insulin stimulated 2-deoxyglucose uptake; cells exposed to monounsaturated fatty acids of the same chain length showed less change. The decrease in insulin binding appeared to be due to changes in both receptor number and affinity. Maximal insulin stimulation of deoxyglucose was decreased with little change in sensitivity to insulin. There was an excellent correlation between the inhibition of both insulin binding and insulin action by the fatty acids tested. Modification of cellular lipids apparently can produce profound effects on both insulin binding and insulin action.