Vestibular dysfunction in the adult CBA/CaJ mouse after lead and cadmium treatment.
Vestibular dysfunction in the adult CBA/CaJ mouse after lead and cadmium treatment.
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DOI：
10.1002/tox.22286
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发表时间：
2017-03
影响因子：
4.5
通讯作者：
Neitzel RL
中科院分区：
文献类型：
--
作者：
Klimpel KE;Lee MY;King WM;Raphael Y;Schacht J;Neitzel RL
前庭系统可以感知位置和运动，其功能障碍表现为运动障碍，眩晕和平衡异常，导致衰弱的心理不适和日常工作困难。虽然在暴露于铅（Pb）的大鼠和暴露于铅和镉（Cd）的人类中发现了前庭功能的下降和缺陷，但没有研究直接检查对内耳前庭器官的病理和病理生理影响。18只年轻成年小鼠通过饮用水（3 mM Pb， 300 μM Cd或对照处理）暴露10周。在治疗前后，他们接受了前庭评估，包括旋转杆性能测试和新型头部稳定性测试，以测量前庭结肠反射。在研究结束时，对胞囊进行免疫组织学分析，观察毛细胞和神经纤维的状况。铅暴露水平增加与空间头部稳定性下降相关；在旋转杆测试中没有发现明显的性能下降。组织学上未见毛细胞及小囊神经纤维损伤。年轻成年CBA/CaJ小鼠能够很好地耐受与职业相关的Pb和Cd暴露，但Pb暴露与头部稳定性降低之间的相关性表明Pb暴露会导致前庭功能下降。
The vestibular system allows the perception of position and motion and its dysfunction presents as motion impairment, vertigo and balance abnormalities, leading to debilitating psychological discomfort and difficulty performing daily tasks. Although declines and deficits in vestibular function have been noted in rats exposed to lead (Pb) and in humans exposed to Pb and cadmium (Cd), no studies have directly examined the pathological and pathophysiological effects upon the vestibular apparatus of the inner ear. Eighteen young adult mice were exposed through their drinking water (3 mM Pb, 300 μM Cd, or a control treatment) for 10 weeks. Before and after treatment, they underwent a vestibular assessment, consisting of a rotarod performance test and a novel head stability test to measure the vestibulocolic reflex. At the conclusion of the study, the utricles were analyzed immunohistologically for condition of hair cells and nerve fibers. Increased levels of Pb exposure correlated with decreased head stability in space; no significant decline in performance on rotarod test was found. No damage to the hair cells or the nerve fibers of the utricle was observed in histology. The young adult CBA/CaJ mouse is able to tolerate occupationally-relevant Pb and Cd exposure well, but the correlation between Pb exposure and reduced head stability suggests that Pb exposure causes a decline in vestibular function.