Relative abundance of saccharides, free amino acids, and other compounds in specific pollen species for source profiling of atmospheric aerosol
Relative abundance of saccharides, free amino acids, and other compounds in specific pollen species for source profiling of atmospheric aerosol
复制标题
DOI：
10.1016/j.scitotenv.2021.149254
复制
发表时间：
2021-08-07
影响因子：
9.8
通讯作者：
Khlystov, Andrey Y.
中科院分区：
文献类型：
--
作者：
Axelrod, Kevin;Samburova, Vera;Khlystov, Andrey Y.
尽管在过去十年中，对生物气溶胶的研究越来越频繁，但对生物气溶胶及其在大气过程中的作用的总体知识广度仍然很低。为了更好地表征新鲜生物气溶胶的化学成分，以便进行来源解析和大气中的示踪，我们选择了几种植物花粉进行详细的化学分析。为此，在美国内华达州里诺市附近购买和采集了不同种类的花粉，以进行进一步提取和详细的化学分析。这些树种包括杨树、玉米、山核桃、豚草、东部白杨、纸桑树、兔子树、苦皮树、短叶松和杰弗里松树。采用气相色谱和超高效液相色谱-质谱仪(GC-MS和UPLC-MS)联用技术对花粉中的糖、游离氨基酸和其他各种极性化合物(如脱水糖和树脂酸)进行了定量分析，以确定特定花粉种的化学组成的差异和细微差别。所有的花粉样品(10个)都普遍含有β-D-果糖、α-D-葡萄糖和β-D-葡萄糖，5个样品中含有蔗糖。松树中也含有D-半乳糖。在所有样品中，总糖占总干重的4.0%至29%。所有样品的总游离氨基酸占总干重的0.29%至15%，其中最常见的氨基酸是脯氨酸。太浩湖地区表层沉积气溶胶的化学组成(包括糖类和氨基酸)与松花粉的相关性最强，表明花粉的化学组成可以用来推断其对当地气溶胶的贡献。(C)2021年爱思唯尔B.V.保留所有权利。
Though studies in bioaerosols are being conducted with increasing frequency over the past decade, the total breadth of knowledge on bioaerosols and their role in atmospheric processes is still minimal. In order to better characterize the chemical composition of fresh biological aerosol for purposes of source apportionment and tracing in the atmosphere, several plant pollen species were selected for detailed chemical analyses. For this purpose, different pollen species were purchased and collected around Reno, Nevada, USA, for further extraction and detailed chemical analysis. These species included aspen, corn, pecan, ragweed, eastern cottonwood, paper mulberry, rabbitbrush, bitterbrush, lodgepole pine, and Jeffrey pine. Saccharides, free amino acids, and various other polar compounds (e.g., anhydrosugars and resin acids) were quantitatively analyzed using gas chromatography and ultra-high performance liquid chromatography coupled with mass spectrometry techniques (GC-MS and UPLC-MS), with the purpose to identify differences and nuances in chemical composition of specific pollen species. The saccharides beta-D-fructose, alpha-D-glucose, and beta-D-glucose were ubiquitously found across all pollen samples (10), and sucrose was found in five samples. D-galactose was also found in pine species. Total saccharides were 4.0 to 29% of total dry weight across all samples. Total free amino acids were 0.29% to 15% of total dry weight across all samples, with the most common amino acid being proline. Chemical profiles (including both saccharides and amino acids) of surface-deposited aerosol in the Lake Tahoe area correlated most closely with pine pollen than other analyzed pollen species, indicating that chemical profiles of pollen can be used to infer its contribution to local aerosols. (C) 2021 Elsevier B.V. All rights reserved.