Regulation of expansin gene expression affects growth and development in transgenic rice plants
Regulation of expansin gene expression affects growth and development in transgenic rice plants
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DOI：
10.1105/tpc.011965
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发表时间：
2003-06-01
期刊：
影响因子：
11.6
通讯作者：
Kende, H
中科院分区：
文献类型：
--
作者：
Choi, DS;Lee, Y;Kende, H
为了研究扩展蛋白在体内的功能，我们产生了表达正义和反义构建体的扩展蛋白基因OsEXP4的转基因水稻植株。在组成型OsEXP4表达的成年植物中，12%的过表达子比平均对照植物更高，88%的过表达子比平均对照植物更矮，并且大多数过表达子至少发育了两片额外的叶子。反义植株比对照植株矮，开花早。在具有诱导型OsEXP4表达的转基因植物中，我们观察到OsEXP4蛋白水平与幼苗生长之间的密切相关性。胚芽鞘和中胚轴长度增加了高达31%和97%，分别在过表达，而在反义幼苗，他们分别下降了28%和43%。幼苗生长的变化是由细胞大小的相应变化引起的，而细胞大小的变化又是细胞壁伸展性改变的函数。我们的研究结果支持这一假设，即膨胀素参与促进生长，介导细胞壁松动。
To investigate the in vivo functions of expansins, we generated transgenic rice plants that express sense and antisense constructs of the expansin gene OsEXP4. In adult plants with constitutive OsEXP4 expression, 12% of overexpressors were taller and 88% were shorter than the average control plants, and most overexpressors; developed at least two additional leaves. Antisense plants were shorter and flowered earlier than the average control plants. In transgenic plants with inducible OsEXP4 expression, we observed a close correlation between OsEXP4 protein levels and seedling growth. Coleoptile and mesocotyl length increased by up to 31 and 97%, respectively, in overexpressors, whereas in antisense seedlings, they decreased by up to 28 and 43%, respectively. The change in seedling growth resulted from corresponding changes in cell size, which in turn appeared to be a function of altered cell wall extensibility. Our results support the hypothesis that expansins are involved in enhancing growth by mediating cell wall loosening.