Establishment of renal cell lines derived from S2 segments of the proximal tubule.
Establishment of renal cell lines derived from S2 segments of the proximal tubule.
复制标题
建立源自近端小管 S2 段的肾细胞系。
DOI：
10.1159/000173395
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
M. Claire
中科院分区：
文献类型：
--
作者：
C. Fauth;D. Chabardès;M. Allaz;M. García;B. Rossier;F. Roch;M. Claire
这项研究的目的是建立来源于明确的肾单位节段的上皮细胞系。原代培养取自解剖的兔肾近端S2段，单层培养。显微注射猴病毒40DNA细胞后观察到永生化，并导致上皮细胞系的发育。这些细胞系在培养中至少保持24代，然后将细胞冷冻。对其中一个细胞系RKPC-2进行了筛选和进一步鉴定。RKPC-2细胞在不渗透的载体上形成穹顶，表明流体和溶质的运输。RKPC-2细胞在包被胶原的滤膜上形成连续的低跨上皮阻力单层。它们能够积累四乙基铵，这是一种有机阳离子；然而，无法测量到显著的跨细胞转运。我们的结论是，该细胞系具有上皮细胞的特征，保持了完整近端小管的某些特性，特别是积累有机阳离子的能力。
The aim of this study was to establish epithelial cell lines derived from defined nephron segments. Primary cultures were prepared from dissected proximal S2 segments of the rabbit kidney, and grown in monolayers. Immortalization was observed after nuclear microinjection of the cells with simian virus 40 DNA and resulted in the development of cell lines of epithelial morphology. These cell lines were maintained in culture for at least 24 passages, then cells were frozen. One of the cell lines, the RKPC-2, was selected and further characterized. RKPC-2 cells formed domes on impermeable supports, indicating fluid and solute transport. RKPC-2 cells formed continuous monolayers of low transepithelial resistance on collagen-coated filters. They were able to accumulate tetraethylammonium, an organic cation; however, no significant transcellular transport could be measured. We conclude that this cell line which shows characteristics of epithelial cells has maintained certain properties of intact proximal tubules, in particular the capacity to accumulate organic cations.