Differences in the regiospecific metabolism of testosterone as catalyzed by variant forms of rabbit microsomal cytochrome P-450 3b.
Differences in the regiospecific metabolism of testosterone as catalyzed by variant forms of rabbit microsomal cytochrome P-450 3b.
复制标题
不同形式的兔微粒体细胞色素 P-450 3b 催化的睾酮区域特异性代谢的差异。
DOI：
10.1016/0006-291x(82)92008-3
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发表时间：
1982
影响因子：
3.1
通讯作者：
Shiverick,KT
中科院分区：
文献类型：
--
作者：
Johnson,EF;Shiverick,KT
从新西兰白兔和 IIIVO J 兔获得的兔微粒体细胞色素 P-450 3b 的高度纯化制剂显示出睾酮区域特异性代谢的动力学差异。来自新西兰白兔的制剂表现出 15 μM 的 6β-羟基化 Km，而从菌株 IIIVO J 中获得的制剂表现出更高的 Km。两种类型的细胞色素 P-450 3b 在睾酮 16α-羟基化方面表现出相似的动力学特性。
Highly purified preparations of rabbit microsomal cytochrome P-450 3b obtained from New Zealand White and IIIVO J rabbits display kinetic differences in the regiospecific metabolism of testosterone. Whereas the preparations from New Zealand White rabbits exhibit a Km of 15 μM for 6β-hydroxylation, those obtained from strain IIIVO J exhibit a much higher Km. The two types of cytochrome P-450 3b exhibit similar kinetic properties for the 16α-hydroxylation of testosterone.