A POLYMORPHIC SYSTEM RELATED TO BUT GENETICALLY INDEPENDENT OF THE CHICKEN MAJOR HISTOCOMPATIBILITY COMPLEX
A POLYMORPHIC SYSTEM RELATED TO BUT GENETICALLY INDEPENDENT OF THE CHICKEN MAJOR HISTOCOMPATIBILITY COMPLEX
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DOI：
10.1007/bf00222464
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发表时间：
1993-02-01
期刊：
影响因子：
3.2
通讯作者：
MILLER, MM
中科院分区：
文献类型：
--
作者：
BRILES, WE;GOTO, RM;MILLER, MM
鸡的主要组织相容性复合体（MHC）的分析表明，血清学定义的单倍型和单倍型之间的不一致性MHC I类和II类基因在Southern杂交的限制性片段模式定义。在从单一血清学定义的B单倍型纯合的鸟类收集的DNA样品中，经常发现Mhc I类和/或II类基因的一种以上的限制性片段模式。这些发现已被解释为以前未检测到的血清学方法的鸡MHC单倍型内的变异性的证据。在这项研究中，一个完整的家系超过三代，观察到的限制性片段模式的异质性被认为是存在的第二个，独立分离的多态性MHC样基因座，指定Rfp-Y的结果。三个等位基因（单倍型），确定在这个新的系统。
Analyses of the major histocompatibility complex (Mhc) in chickens have shown inconsistencies between serologically defined haplotypes and haplotypes defined by the restriction fragment patterns of Mhc class I and class II genes in Southern hybridizations. Often more than one pattern of restriction fragments for Mhc class I and/or class II genes has been found among DNA samples collected from birds homozygous for a single serologically defined B haplotype. Such findings have been interpreted as evidence for variability within the Mhc haplotypes of chickens not detected previously with serological methods. In this study of a fully pedigreed family over three generations, the heterogeneity observed in restriction fragment patterns was found to be the result of the presence of a second, independently segregating polymorphic Mhc-like locus, designated Rfp-Y. Three alleles (haplotypes) are identified in this new system.