Vaccination with recombinant oncosphere antigens reduces the susceptibility of sheep to infection with Taenia multiceps.
Vaccination with recombinant oncosphere antigens reduces the susceptibility of sheep to infection with Taenia multiceps.
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DOI：
10.1016/j.ijpara.2007.11.006
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发表时间：
2008-07
影响因子：
4
通讯作者：
Lightowlers MW
中科院分区：
文献类型：
--
作者：
Gauci C;Vural G;Oncel T;Varcasia A;Damian V;Kyngdon CT;Craig PS;Anderson GA;Lightowlers MW
多头带绦虫是一种绦虫寄生虫，其幼虫阶段在绵羊、山羊和牛的大脑中包囊，导致通常致命的情况。这种寄生虫也会引起人畜共患传染病。羊带绦虫和其他带绦虫的重组六钩蚴疫苗抗原的同源物在T。多头的相关T. multiceps基因和编码mRNA的克隆揭示了基因和蛋白质中的保守特征。霸王命名为Tm16和Tm18的多头六钩蚴蛋白含有预测的分泌信号和纤连蛋白III型结构域。重组Tm16和Tm18蛋白在大肠杆菌中以GST融合蛋白的形式表达。用Quil A佐剂配制的抗原在绵羊的疫苗试验中进行了测试。该抗原可刺激绵羊产生抗弓形虫攻击感染的免疫力。多头卵9只对照羊中有5只死于T.而20只接种疫苗的动物中没有一只由于寄生虫攻击而死亡（P = 0.001）。此外，接种Tm16蛋白，或Tm16加Tm18，诱导显着的保护作用，对寄生虫包囊在大脑中的攻击感染的结果（P = 0.023，P = 0.015，分别）。接种动物中特异性血清抗体水平与部分接种动物中存在或不存在寄生虫或寄生虫数量之间无明显关系。我们相信这项研究是第一个描述的重组疫苗相关的调查，为T。多头的所鉴定的重组六钩蚴抗原可能有助于开发有效的抗T.绵羊多头感染。它们提高了与细粒棘球绦虫EG95抗原联合疫苗的开发潜力，用于预防T。以及防止囊状棘球蚴病的传播。
Taenia multiceps is a cestode parasite, the larval stage of which encysts in the brain of sheep, goats and cattle causing an often fatal condition. The parasite also causes zoonotic infections in humans. Homologues of the recombinant oncosphere vaccine antigens from Taenia ovis and other Taenia species were identified in T. multiceps. Sequencing of the associated T. multiceps genes and cloning of the encoding mRNA has revealed conserved features in the genes and proteins. The T. multiceps oncosphere proteins, designated Tm16 and Tm18, contain a predicted secretory signal and fibronectin type III domain. The recombinant Tm16 and Tm18 proteins were successfully expressed in Escherichia coli as fusion proteins with GST. The antigens, formulated with Quil A adjuvant, were tested in a vaccine trial in sheep. The antigens stimulated immunity in sheep against challenge infection with T. multiceps eggs. Five of nine control sheep died due to a challenge infection with T. multiceps whereas none of 20 vaccinated animals died as a result of the parasite challenge (P = 0.001). In addition, vaccination with the Tm16 protein, or Tm16 plus Tm18, induced significant protection against the number of parasites encysting in the brain as a result of the challenge infection (P = 0.023, P = 0.015, respectively). No clear relationship was apparent between the level of specific serum antibody in vaccinated animals and either the presence or absence of parasites or the number of parasites that occurred in some of the vaccinated animals. We believe this study is the first description of recombinant vaccine-related investigations for T. multiceps. The recombinant oncosphere antigens identified may allow development of effective vaccination strategies against T. multiceps infection in sheep. They raise the potential for the development of a combined vaccine with the Echinococcus granulosus EG95 antigen for prevention of T. multiceps as well as preventing the transmission of cystic hydatid disease.