PpWRKY45 is involved in methyl jasmonate primed disease resistance by enhancing the expression of jasmonate acid biosynthetic and pathogenesis-related genes of peach fruit
PpWRKY45 is involved in methyl jasmonate primed disease resistance by enhancing the expression of jasmonate acid biosynthetic and pathogenesis-related genes of peach fruit
复制标题
PpWRKY45通过增强桃果实茉莉酸生物合成和发病机制相关基因的表达参与茉莉酸甲酯引发的抗病性
DOI：
10.1016/j.postharvbio.2020.111390
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发表时间：
2021-02-01
影响因子：
7
通讯作者：
Zheng, Yonghua
中科院分区：
文献类型：
--
作者：
Ji, Nana;Wang, Jing;Zheng, Yonghua
茉莉酸甲酯（MeJA）和WRKY转录因子（TF）被证明发挥重要的防御功能。关于WRKY TF与MeJA引发的桃果实抗根霉防御的研究仍处于起步阶段。研究了MeJA对桃采后软腐病的诱导抗性模式及WRKY TF的参与作用。结果表明，MeJA在10 μ M浓度下显著降低了采后软腐病的发病率和病斑直径。在20 ℃下储存60 h期间stolonifer感染。此外，MeJA处理促进了CHI和GLU的活性，并提高了PpLOX、PpAOS和PpOPR 3的表达水平。MeJA预处理和接种腐霉后，PpCHI、PpGLU、PpPR-like、PpLOX、PpAOS和PpOPR 3基因的表达量显著增加，表明MeJA诱导了桃对腐霉的特异性启动防御。此外，一个MeJA相关的转录因子PpWRKY 45被鉴定和表征为核定位蛋白，其可以通过与启动子中的W-box元件结合来激活PpCHI、PpGLU、PpPR样、PpLOX、PpAOS和PpOPR 3的表达。这些结果表明PpWRKY 45参与了MeJA引发的针对R.通过激活桃果实JA生物合成基因和PR基因，
Methyl jasmonate (MeJA) and WRKY transcription factors (TFs) are documented to exert vital defensive functions. Research concerning WRKY TFs together with MeJA-primed defense against Rhizopus stolonifer in peach fruit is still in its infancy. In the present study, the mode of MeJA on inducing resistance against soft rot caused by Rhizopus stolonifer in peaches during the postharvest storage and the involvement of WRKY TFs were investigated, and the results manifested that MeJA at 10 mu M significantly reduced disease occurrence and lesion diameter after R. stolonifer infection during 60 h of storage at 20 degrees C. Moreover, the MeJA treatment promoted the activity of CHI and GLU and elevated the expression levels of PpLOX, PpAOS and PpOPR3. More importantly, the expression of PpCHI, PpGLU, PpPR-like, PpLOX, PpAOS and PpOPR3 were substantially and rapidly elevated in the peach fruit that pretreated with MeJA and inoculated with R.stolonifer, indicating that MeJA stimulated a specific priming defense against Rhizopus rot in peaches. Furthermore, a MeJA-related transcription factor PpWRKY45 was identified and characterized as a nucleus-localized protein that could activate the expression of PpCHI, PpGLU, PpPR-like, PpLOX, PpAOS and PpOPR3 by binding to W-box elements in their promoters. These results indicate that PpWRKY45 is involved in MeJA-primed defense against R. stolonifer by activating JA biosynthetic and PR genes of peach fruit.