SKELETAL UNLOADING IN RAT DECREASES PROLIFERATION OF RAT BONE AND MARROW-DERIVED OSTEOBLASTIC CELLS
SKELETAL UNLOADING IN RAT DECREASES PROLIFERATION OF RAT BONE AND MARROW-DERIVED OSTEOBLASTIC CELLS
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DOI：
10.1152/ajpendo.1993.264.5.e790
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发表时间：
1993-05-01
影响因子：
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通讯作者：
MARIE, PJ
中科院分区：
文献类型：
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作者：
MACHWATE, M;ZERATH, E;MARIE, PJ
在尾部悬吊大鼠模型上观察骨骼去负荷对成骨细胞的影响。与对照组相比，后肢抬高14天引起骨量减少，胫骨干骺端骨形成的组织形态计量学指标降低，血浆骨钙素和碱性磷酸酶（ALP）水平降低。从悬浮胫骨的骨内膜骨表面分离的成骨细胞的体外增殖在培养的第2天和第4天分别比对照组减少42%和31%，如[H-3]胸苷标记和细胞数所示。ALP阳性骨髓基质细胞的增殖也减少了20-24%，在1和2天的培养。然而，ALP活性在骨源性细胞和骨髓基质细胞中没有不同的卸载和对照组，骨细胞合成骨钙素，骨粘连蛋白，和I型或III型胶原的数量是相同的两组。结果表明，骨骼卸载诱导的骨形成抑制与骨内膜骨表面和骨髓基质中沿着存在的推定成骨细胞前体细胞的增殖减少有关。
The effects of skeletal unloading on osteoblastic cells were evaluated in tail-suspended rats. Hindlimb elevation for 14 days induced osteopenia, decreased histomorphometric indexes of bone formation in tibial metaphysis, and reduced plasma osteocalcin and alkaline phosphatase (ALP) levels compared with controls. The in vitro proliferation of osteoblastic cells isolated from the endosteal bone surface of suspended tibias was decreased by 42 and 31% at 2 and 4 days of culture, respectively, compared with controls, as shown by [H-3]thymidine labeling and cell-number. The proliferation of ALP-positive marrow stromal cells was also decreased by 20-24% at 1 and 2 days of culture. However, ALP activity in bone-derived cells and marrow stromal cells was not different in unloaded and control rats, and the number of bone cells synthesizing osteocalcin, osteonectin, and type I or type III collagen was identical in the two groups. The results indicate that the inhibition of bone formation induced by skeletal unloading is related to a decreased proliferation of putative osteoblast precursor cells present along the endosteal bone surface and in the marrow stroma.