Nitric oxide synthase activity in the pregnant uterus decreases at parturition.
Nitric oxide synthase activity in the pregnant uterus decreases at parturition.
复制标题
妊娠子宫中的一氧化氮合酶活性在分娩时降低。
DOI：
10.1006/bbrc.1993.1776
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发表时间：
1993
影响因子：
3.1
通讯作者：
Riemer,RK
中科院分区：
文献类型：
--
作者：
Natuzzi,ES;Ursell,PC;Harrison,M;Buscher,C;Riemer,RK
介导子宫活动从怀孕期间的静止状态到分娩时的活跃产程变化的机制尚不清楚。一氧化氮（NO）是平滑肌松弛的有效介质，其在子宫中的存在是本报告的主题。NADPH-diaphorase染色法检测了大鼠妊娠子宫神经、血管和蜕膜中一氧化氮合酶（NOS）的活性。分娩时固定的子宫组织显示NOS明显减少。对孕妇和产妇子宫亚细胞部分NOS活性的定量分析显示，NOS存在于子宫匀浆的细胞质室和膜质室中。在这两个细胞亚组中，酶活性从怀孕到足月显著下降。我们认为NOS存在于子宫内的多个结构中。它出现在两个细胞区室表明子宫内可能存在不止一种形式的NOS。分娩时NOS活性的降低表明NO可能有助于维持妊娠期间子宫收缩静止。
The mechanisms that mediate changes in uterine activity from a quiescent state during pregnancy to active labor at parturition are unknown. Nitric oxide (NO), a potent mediator of smooth muscle relaxation, and its presence in the uterus is the subject of this report. Nitric oxide synthase (NOS) activity was demonstrated in nerves, blood vessels and decidua of gravid rat uterus by the NADPH-diaphorase staining method. Uterine tissue fixed during labor demonstrated markedly less NOS. Quantitation of NOS activity in subcellular fractions of pregnant and laboring uterus revealed its presence in both the cytosolic and the membranous compartments of uterine homogenates. In both cellular subfractions the enzyme activity decreased significantly from pregnancy to term. We conclude NOS is present in multiple structures within the uterus. Its presence in two cellular compartments suggests more than one form of NOS may be present in the uterus. Reduction in NOS activity at parturition suggests NO may contribute to the maintenance of uterine contractile quiescence during gestation.