Short tandem repeat profiling provides an international reference standard for human cell lines
Short tandem repeat profiling provides an international reference standard for human cell lines
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DOI：
10.1073/pnas.121616198
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发表时间：
2001-07-03
影响因子：
11.1
通讯作者：
Debenham, PG
中科院分区：
文献类型：
--
作者：
Masters, JR;Thomson, JA;Debenham, PG
细胞系之间的交叉污染是科学上错误陈述的长期和频繁的原因。国家检测服务机构的估计表明，多达36%的细胞系与声称的来源或物种不同。为了测试细胞系鉴定的标准方法，对来自世界各地银行和研究机构的253个人类细胞系进行了简短的分析：串联重复图谱。短串联重复序列图谱是一种简单的数字代码，可在实验室之间重现，价格低廉，并可为每一种细胞系提供国际参考标准。如果细胞系的DMA图谱在国际上被接受并被要求，由于交叉污染而造成的科学错误陈述可以在很大程度上被消除。
Cross-contamination between cell lines is a longstanding and frequent cause of scientific misrepresentation. Estimates from national testing services indicate that up to 36% of cell lines are of a different origin or species to that claimed. To test a standard method of cell line authentication, 253 human cell lines from banks and research institutes worldwide were analyzed by short: tandem repeat profiling. The short tandem repeat profile is a simple numerical code that is reproducible between laboratories, is inexpensive, and can provide an international reference standard for every cell line. If DMA profiling of cell lines is accepted and demanded internationally, scientific misrepresentation because of cross-contamination can be largely eliminated.