Induced activity of adenine phosphoribosyltransferase (APRT) in iron-deficiency barley roots: a possible role for phytosiderophore production.
Induced activity of adenine phosphoribosyltransferase (APRT) in iron-deficiency barley roots: a possible role for phytosiderophore production.
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缺铁大麦根中腺嘌呤磷酸核糖转移酶（APRT）的诱导活性：植物铁载体产生的可能作用。
DOI：
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发表时间：
2000
影响因子：
6.9
通讯作者：
S. Mori
中科院分区：
文献类型：
--
作者：
R. N. Itai;Kazuya Suzuki;H. Yamaguchi;H. Nakanishi;N. Nishizawa;Etsuro Yoshimura;S. Mori
为了分离禾本科植物对缺铁胁迫响应的相关基因，从大麦（Hordeum vulgare L.）根基于该蛋白的部分氨基酸序列，从从Fe缺乏的大麦根制备的cDNA文库中克隆了编码腺嘌呤磷酸核糖基转移酶（APRT：EC2.4.2.7）的cDNA（HvAPT 1）。Southern分析表明，大麦中至少有两个编码APRT的基因。缺铁增加了HvAPT 1在大麦根中的表达，并且向缺铁植物再供应铁迅速否定了HvAPT 1 mRNA的增加。对HvAPT 1-sGFP融合蛋白在烟草BY-2细胞中的定位分析表明，来自HvAPT 1的蛋白定位于细胞质中。与北方分析结果一致，缺铁显著提高了大麦根中APRT的酶活性。在其他禾本科植物如黑麦、玉米和水稻的根中也观察到铁缺乏对APRT活性的诱导。相反，在非禾本科植物烟草的根中未观察到诱导。禾本科植物通常在缺铁条件下在根中合成麦根酸家族植物铁载体（MAs）。在本文中，一个可能的作用HvAPT 1在相关的腺嘌呤补救甲硫氨酸循环中的MA的生物合成进行了讨论。
To isolate the genes involved in the response of graminaceous plants to Fe-deficient stress, a protein induced by Fe-deficiency treatment was isolated from barley (Hordeum vulgare L.) roots. Based on the partial amino acid sequence of this protein, a cDNA (HvAPT1) encoding adenine phosphoribosyltransferase (APRT: EC 2.4.2.7) was cloned from a cDNA library prepared from Fe-deficient barley roots. Southern analysis suggested that there were at least two genes encoding APRT in barley. Fe deficiency increased HvAPT1 expression in barley roots and resupplying Fe to the Fe-deficient plants rapidly negated the increase in HvAPT1 mRNA. Analysis of localization of HvAPT1-sGFP fusion proteins in tobacco BY-2 cells indicated that the protein from HvAPT1 was localized in the cytoplasm of cells. Consistent with the results of Northern analysis, the enzymatic activity of APRT in barley roots was remarkably increased by Fe deficiency. This induction of APRT activity by Fe deficiency was also observed in roots of other graminaceous plants such as rye, maize, and rice. In contrast, the induction was not observed to occur in the roots of a non-graminaceous plant, tobacco. Graminaceous plants generally synthesize the mugineic acid family phytosiderophores (MAs) in roots under Fe-deficient conditions. In this paper, a possible role of HvAPT1 in the biosynthesis of MAs related to adenine salvage in the methionine cycle is discussed.
DOI：
10.1073/pnas.85.23.8998
发表时间：
1988-12-01
影响因子：
11.1
作者：
FROHMAN, MA;DUSH, MK;MARTIN, GR
通讯作者：
MARTIN, GR