Tomato LrgB regulates heat tolerance and the assimilation and partitioning of carbon
Tomato LrgB regulates heat tolerance and the assimilation and partitioning of carbon
复制标题
番茄 LrgB 调节耐热性以及碳的同化和分配
DOI：
10.1016/j.plantsci.2018.06.001
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发表时间：
2018-09-01
期刊：
影响因子：
5.2
通讯作者：
Zhang，Junhong
中科院分区：
文献类型：
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作者：
Zhang，Shiwen;Ai，Guo;Zhang，Junhong
极端和持续高温对植物生长的影响日益突出。热休克同源70-kDa蛋白在植物耐热性中起重要作用。在这项研究中，我们鉴定和表征了番茄同源物flrgb (SlLrgB)，并证明它与Hsc70.1相互作用。与其他编码叶绿体定位蛋白的基因类似，sllrgbi的表达在绿色组织中上调，并受到热休克的抑制。利用转基因植物进行的功能分析表明，sllrgb参与叶绿素代谢。sllrgbled的过表达和RNA干扰抑制均导致叶片褪绿，使成熟果实株高降低，大小减小，色素水平降低。然而，sllrgb - rnai系的淀粉含量显著增加，耐热性明显提高。通过VIGS下调hsc70.1的表达，导致番茄生长迟缓，叶片褪绿，sllrgb的表达增加。基于这些数据，我们认为sllrg调节叶绿素代谢和碳的同化和分配。我们还认为Hsc70.1和SlLrgB有助于耐热性，而athsc70.1负向调节essllrgb。
The impact of extreme and sustained high temperatures on plant growth has become increasingly prominent. Heat shock cognate 70-kDa proteins play an important role in plant heat tolerance. In this study, we identified and characterized the tomato ortholog ofLrgB(SlLrgB), and demonstrate that it interacts with Hsc70.1. Similar to other genes that encode chloroplast-localized proteins, the expression ofSlLrgBis upregulated in green tissues and suppressed by heat shock. Functional analyses utilizing transgenic plants indicate thatSlLrgBcontributes to chlorophyll metabolism. Both the overexpression and the RNA interference-mediated suppression ofSlLrgBled to chlorotic leaves, reduced plant height, smaller size and decreases in pigment levels in ripening fruits. However, the starch levels in theSlLrgB-RNAi lines were significantly increased and the heat tolerance ofSlLrgB-RNAi was obvious elevated. Downregulating the expression ofHsc70.1by VIGS in tomato led to retarded growth, chlorotic leaves, and increased expression ofSlLrgB.Based on these data, we suggest thatSlLrgBregulates chlorophyll metabolism and the assimilation and partitioning of carbon. We also suggest that Hsc70.1 and SlLrgB contribute to heat tolerance and thatHsc70.1negatively regulatesSlLrgB.