Risk factors of different hemoplasma species infections in cats.
Risk factors of different hemoplasma species infections in cats.
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DOI：
10.1186/s12917-017-0953-3
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发表时间：
2017-02-16
影响因子：
2.6
通讯作者：
Hartmann K
中科院分区：
文献类型：
--
作者：
Bergmann M;Englert T;Stuetzer B;Hawley JR;Lappin MR;Hartmann K
血浆种属（属）在全球范围内普遍引起猫感染。然而，关于感染风险因素的数据有限。这项研究的目的是确定流行的血浆种。在德国南部的猫感染，并评估与感染相关的风险因素。从479只猫的血液样本中提取DNA，这些猫因各种原因被送到不同的兽医医院。用PCR方法扩增猫血浆DNA。直接测序用于确认所有纯化的扩增子，并与GenBank中报道的血浆序列进行比较。评价了与猫的年龄、性别、饲养条件、猫白血病病毒（FeLV）和猫免疫缺陷病毒（FIV）状态相关的结果。总体血浆患病率为9.4%（45/479; 95% CI：7.08-12.36）。（M.）haemominutum'（Mhm）DNA的扩增结果表明，M. haemofelis（2）和M. 1份样本的血吸虫病。在来自多猫家庭的猫、户外猫以及FIV感染猫和流产FeLV感染猫中，血浆感染风险显著较高，但在进行性或消退性FeLV感染猫中不存在。Mhm感染在德国南部的猫中很常见。多猫家庭中的较高患病率以及与FeLV感染的相关性可能反映了猫之间直接传播的可能性。户外活动、男性性别和FIV感染是可能与攻击性互动和接触病媒相关的额外风险因素。
Hemoplasma species (spp.) commonly cause infections in cats worldwide. However, data on risk factors for infections are limited. The aim of this study was to determine the prevalence of hemoplasma spp. infections in cats in Southern Germany and to assess risk factors associated with infection. DNA was extracted from blood samples of 479 cats presented to different veterinary hospitals for various reasons. DNA of feline hemoplasmas was amplified by use of a previously reported PCR assay. Direct sequencing was used to confirm all purified amplicons and compared to hemoplasma sequences reported in GenBank. Results were evaluated in relation to the age, sex, housing conditions, feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) status of the cats. The overall hemoplasma prevalence rate was 9.4% (45/479; 95% CI: 7.08–12.36). ‘Candidatus Mycoplasma (M.) haemominutum’ (Mhm) DNA was amplified from 42 samples, M. haemofelis from 2, and M. haemocanis from 1 sample. There was a significantly higher risk of hemoplasma infection in cats from multi-cat households, in outdoor cats, as well as in cats with FIVinfection and in cats with abortive FeLV infection, but not in cats with progressive or regressive FeLV infection. Mhm infection is common in cats in Southern Germany. Higher prevalence in multi-cat households and associations with FeLV infection likely reflect the potential for direct transmission amongst cats. Outdoor access, male gender, and FIV infection are additional risk factors that might relate to aggressive interactions and exposure to vectors.