Molecular typing of Brucella species isolates from livestock and human
Molecular typing of Brucella species isolates from livestock and human
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家畜和人类布鲁氏菌分离株的分子分型
DOI：
10.1007/s11250-011-9886-1
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发表时间：
2011
影响因子：
1.7
通讯作者：
K. Prabhudas
中科院分区：
文献类型：
--
作者：
M. Nagalingam;R. Shome;V. Balamurugan;B. Shome;Krishnamsetty NarayanaRao;Vivekānanda;S. Isloor;K. Prabhudas
尽管在不同种属的布鲁氏菌中观察到宿主特异性，但跨越动物宿主边界可能随时发生。本研究采用布鲁斯梯状PCR和羊流产-羊-猪（AMOS）PCR方法对47株来自印度的布鲁氏菌分离株进行了鉴定，以了解布鲁氏菌病的流行病学。其中牛种布鲁氏菌28株，羊种布鲁氏菌14株，猪种布鲁氏菌5株。进一步的AMOS PCR分析表明，所有的B.流产杆菌分离株属于生物变型1、2或4中的任一种;属于5个B中的任一种。猪分离株中，三个属于生物变型1，两个属于生物变型2、3、4或5中的任一种。虽然这种多重布鲁斯梯状PCR可用于区分所有布鲁氏菌属种，但需要进行详细研究以进一步在准确的生物型水平上表征分离株。
Although host specificity has been observed in different species of Brucella, crossing the animal host boundary is likely to occur at any time. In this study, Bruce ladder PCR and abortus–melitensis–ovis–suis (AMOS) PCR assays were used to characterize 47 Brucella isolates from Indian origin in order to know exact species for understanding epidemiology of brucellosis. Out of them, 28, 14, and 5 isolates were found to be Brucella abortus, Brucella melitensis, and Brucella suis, respectively. Further analysis by AMOS PCR has identified that all the B. abortus isolates belong to any one of the biovar 1, 2, or 4; of the five B. suis isolates, three belong to biovar 1 and two belong to any one of the biovar 2, 3, 4, or 5. Although this multiplex Bruce ladder PCR is useful in differentiating all Brucella species, elaborate study is required to further characterize the isolates at exact biovar level.