Jejunal transport properties of piebald mouse model for Hirschsprung's disease.
Jejunal transport properties of piebald mouse model for Hirschsprung's disease.
复制标题
先天性巨结肠花斑小鼠模型的空肠转运特性。
DOI：
10.1152/ajpgi.1980.239.2.g123
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
A. R. Cooke
中科院分区：
文献类型：
--
作者：
H. Cooke;H. J. Henning;J. Wood;A. R. Cooke
在没有电化学梯度的情况下，测定了3-4周龄正常(N)小鼠或先天性(P)或诱导(I)巨结肠小鼠的空肠段丙氨酸、蛋氨酸和钠的单向通量。P和I小鼠的短路电流分别为286.8+/-16.3mA/cm~2和302.1+/-32.6mA/cm~2，约为N小鼠的1.5-2倍。P组和I组小鼠的组织电导平均值分别为42.3+/-0.6和39.3+/-3.4ms/cm2，是N组的两倍。P组小鼠的净钠吸收是N组的2-3倍，添加丙氨酸可增加P组小鼠的净钠吸收。丙氨酸和蛋氨酸被积极吸收，丙氨酸净通量是正常小鼠的2-3倍。P和I组小鼠的单向丙氨酸内流增加。N和P小鼠的Jmax分别为9.7+/-2.6和14.2+/-1.0微米×厘米~(-2)×小时~(-1)。这些结果表明，巨结肠小鼠的空肠粘膜转运特性与正常小鼠不同。巨结肠小鼠对丙氨酸和钠的吸收速度比正常小鼠更快，氨基酸吸收速度的提高可以用丙氨酸内流的增加来解释，这可能是因为活跃的运输载体的数量增加了。
Unidirectional fluxes of alanine, methionine, and Na were measured in jejunal segments from 3- to 4-wk-old normal (N) mice or mice with congenital (P) or induced (I) megacolon in the absence of electrochemical gradients. Short-circuit currents were 286.8 +/- 16.3 and 302.1 +/- 32.6 mu A/cm2 for P and I mice, respectively, and were approximately 1.5-2 times greater than in N mice. Tissue conductances averaged 42.3 +/- 0.6 and 39.3 +/- 3.4 mS/cm2 for P and I mice, respectively, and were twice those found in N mice. Net Na absorption was 2-3 times greater in P than N mice and was increased by addition of alanine. Alanine and methionine were actively absorbed and the net fluxes of alanine were 2-3 times greater in p and I mice than in normals. Unidirectional alanine influx was enhanced in P and I mice. Jmax of 9.7 +/- 2.6 and 14.2 +/- 1.0 micro M x cm-2 x h-1 were obtained for N and P mice, respectively. These results show that the transport properties of jejunal mucosa of mice with megacolon differ from those of N mice. Mice with megacolon absorb alanine and Na at a greater rate than N mice and the enhanced amino acid absorptive rate can be explained by an increased alanine influx, probably due to an increased number of active transport carriers.