Quantification of brain glycogen concentration and turnover through localized 13C NMR of both the C1 and C6 resonances.
Quantification of brain glycogen concentration and turnover through localized 13C NMR of both the C1 and C6 resonances.
复制标题
通过 C1 和 C6 共振的局部 13C NMR 定量脑糖原浓度和周转率。
DOI：
10.1002/nbm.1460
复制
发表时间：
2010
影响因子：
2.9
通讯作者：
Gruetter,Rolf
中科院分区：
文献类型：
--
作者：
vanHeeswijk,RuudB;Morgenthaler,FlorenceD;Xin,Lijing;Gruetter,Rolf
We have recently shown that at isotopic steady state13C NMR can provide a direct measurement of glycogen concentration changes, but that the turnover of glycogen was not accessible with this protocol. The aim of the present study was to design, implement and apply a novel dual‐tracer infusion protocol to simultaneously measure glycogen concentration and turnover. After reaching isotopic steady state for glycogen C1 using [1‐13C] glucose administration, [1,6‐13C2] glucose was infused such that isotopic steady state was maintained at the C1 position, but the C6 position reflected13C label incorporation. To overcome the large chemical shift displacement error between the C1 and C6 resonances of glycogen, we implemented 2D gradient based localization using the Fourier series window approach, in conjunction with time‐domain analysis of the resulting FIDs using jMRUI. The glycogen concentration of 5.1 ± 1.6 mM measured from the C1 position was in excellent agreement with concomitant biochemical determinations. Glycogen turnover measured from the rate of label incorporation into the C6 position of glycogen in theα‐chloralose anesthetized rat was 0.7 µmol/g/h. Copyright © 2009 John Wiley & Sons, Ltd.
