Knockdown of long non-coding RNA PVT1 induces apoptosis and cell cycle arrest in clear cell renal cell carcinoma through the epidermal growth factor receptor pathway
Knockdown of long non-coding RNA PVT1 induces apoptosis and cell cycle arrest in clear cell renal cell carcinoma through the epidermal growth factor receptor pathway
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长链非编码RNA PVT1的敲低通过表皮生长因子受体途径诱导透明细胞肾细胞癌的细胞凋亡和细胞周期停滞
DOI：
10.3892/ol.2018.8315
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发表时间：
2018-05-01
期刊：
影响因子：
2.9
通讯作者：
Xie, Wenlian
中科院分区：
文献类型：
--
作者：
Li, Weicong;Zheng, Zaosong;Xie, Wenlian
近年来，长链非编码rna （lncRNAs）在癌症研究中的重要性得到了证实。lncRNA Pvt1癌基因（非蛋白编码）（Pvt1）在各种癌症类型中被发现上调。本研究的目的是探讨PVT1在透明细胞肾细胞癌（ccRCC）中的功能。应用逆转录-定量聚合酶链反应分析PVT1在ccRCC中的表达，发现PVT1在ccRCC组织中的表达较正常癌旁组织上调。接下来，我们验证了来自The Cancer Genome Atlas数据集的PVT1表达，并发现PVT1的高表达与晚期疾病和不良预后相关。此外，PVT1的敲低通过增加聚ADP核糖聚合酶和bcl -2相关X蛋白的表达诱导细胞凋亡，并通过降低cyclin D1的表达促进细胞周期阻滞在G1期。机制研究表明，PVT1部分通过激活表皮生长因子受体途径促进ccRCC的进展。总之，本研究结果表明PVT1具有致癌功能，可能是ccRCC的生物标志物和治疗靶点。
Previous years have witnessed the importance of long non-coding RNAs (lncRNAs) in cancer research. The lncRNA Pvt1 oncogene (non-protein coding) (PVT1) was revealed to be upregulated in various cancer types. The aim of the present study was to investigate the function of PVT1 in clear cell renal cell carcinoma (ccRCC). The expression of PVT1 in ccRCC was analyzed using reverse transcription-quantitative polymerase chain reaction, and it was revealed that PVT1 expression was upregulated in ccRCC tissues compared with that in normal adjacent tissues. Next, PVT1 expression from The Cancer Genome Atlas datasets was validated, and it was also revealed that the high expression of PVT1 was associated with advanced disease stage and a poor prognosis. Furthermore, the knockdown of PVT1 induced apoptosis by increasing the expression of poly ADP ribose polymerase and Bcl-2-associated X protein, and promoted cell cycle arrest at the G1 phase by decreasing the expression of cyclin D1. Study of the mechanism involved indicated that PVT1 promoted the progression of ccRCC partly through activation of the epidermal growth factor receptor pathway. Altogether, the results of the present study suggested that PVT1 serves oncogenic functions and may be a biomarker and therapeutic target in ccRCC.