FUNCTIONAL-CHARACTERIZATION OF THE CELLS IN CHRONIC NEUTROPHILIC LEUKEMIA
FUNCTIONAL-CHARACTERIZATION OF THE CELLS IN CHRONIC NEUTROPHILIC LEUKEMIA
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DOI：
10.1002/ajh.2830120208
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发表时间：
1982-01-01
影响因子：
12.8
通讯作者：
WONG, D
中科院分区：
文献类型：
--
作者：
DOTTEN, DA;PRUZANSKI, W;WONG, D
慢性嗜中性粒细胞性白血病（CNL）的中性粒细胞的EM和光学显微镜没有发现与正常成熟中性粒细胞的差异。CNL细胞的功能特性与正常细胞相比有显著差异。CNL中性粒细胞在次优条件下的存活率要低得多。自体血清进一步降低了它们的存活率，并通过正常人血清进行校正。CNL细胞表现出非常活跃的吞噬作用，但其杀菌活性在次优条件下降低。溶菌酶和β-溶菌酶的总含量与正常中性粒细胞相比，CNL细胞中的葡萄糖醛酸酶较低，但这些酶从刺激的细胞中的释放比正常高得多。这一观察结果与明显的溶酶体不稳定性相一致。在CFU-C [培养物中的集落形成单位]测定中，来自患者外周血和骨髓的细胞显示过度生长。观察到CNL细胞对冷凝集素、SLE血清和CSF集落刺激因子的细胞毒性活性的显著敏感性，这可能意味着与正常细胞相比，CNL中性粒细胞的膜结构存在定性和/或定量差异。
EM and light microscopy of neutrophils from chronic neutrophilic leukemia (CNL) did not reveal differences from normal mature neutrophils. Functional characterization of CNL cells showed marked differences when compared to normal cells. CNL neutrophils were much less viable in suboptimal conditions. Their survival was further reduced by autologous serum and was corrected by normal human serum. CNL cells showed very active phagocytosis, but their bactericidal activity was reduced in suboptimal conditions. The total content of lysozyme and .beta.-glucuronidase was lower in CNL cells compared to normal neutrophils, but the release of these enzymes from stimulated cells was much higher than normal. This observation is compatible with a marked lysosomal lability. Cells from the patients'' peripheral blood and bone marrow showed excessive growth in CFU-C [colony forming units in culture] assays. Marked susceptibility of CNL cells to cytotoxic activity of cold agglutinins, SLE sera and CSF colony stimulating factors was observed and may signify qualitative and/or quantitative differences in the membrane structure of CNL neutrophils, as compared to normal cells.