IDENTIFICATION OF A 7 TRANSMEMBRANE HELIX RECEPTOR FOR CORTICOTROPIN-RELEASING FACTOR AND SAUVAGINE IN MAMMALIAN BRAIN
IDENTIFICATION OF A 7 TRANSMEMBRANE HELIX RECEPTOR FOR CORTICOTROPIN-RELEASING FACTOR AND SAUVAGINE IN MAMMALIAN BRAIN
复制标题
DOI：
10.1016/0896-6273(93)90230-o
复制
发表时间：
1993-12-01
期刊：
影响因子：
16.2
通讯作者：
ROSENFELD, MG
中科院分区：
文献类型：
--
作者：
CHANG, CP;PEARSE, RV;ROSENFELD, MG
我们已经鉴定和表征了编码一种新型受体的cDNA，该受体是一种独特的七跨膜螺旋G（s）偶联受体。该受体介导响应于生理浓度的促肾上腺皮质激素释放因子（CRF）和相关的青蛙皮肤肽sauvagine的细胞内cAMP水平的配体依赖性刺激。CRF受体mRNA在脑、脑垂体和其他器官中的表达模式与经典CRF受体的预测模式精确对应，表明该受体可介导CRF对行为、应激和稳态的已知生物学效应。选择性剪接事件产生第二个相对丰富的基因产物，以不同的个体发育模式表达。这些发现有助于确定一种重要神经肽的受体。
We have identified and characterized cDNAs encoding a novel receptor that is a member of a distinct class of seven transmembrane helix, G(s)-coupled receptors. This receptor mediates ligand-dependent stimulation of intracellular cAMP levels in response to physiologic concentrations of corticotropin-releasing factor (CRF) and to the related frog skin peptide, sauvagine. The pattern of CRF receptor mRNA expression in the brain, pituitary gland, and other organs corresponds precisely to that predicted for the classic CRF receptor, suggesting that this receptor serves to mediate the known biological effects of CRF on behavior, stress, and homeostasis. Alternative splicing events generate a second, relatively abundant gene product expressed in a distinct ontogenic pattern. These findings serve to identify the receptor for an important neuropeptide.