Cytokinin and auxin regulates WUS induction and inflorescence regeneration in vitro in Arabidopsis
Cytokinin and auxin regulates WUS induction and inflorescence regeneration in vitro in Arabidopsis
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细胞分裂素和生长素在拟南芥体外调节 WUS 诱导和花序再生
DOI：
10.1007/s00299-010-0879-8
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发表时间：
2010-08-01
影响因子：
6.2
通讯作者：
Zhang, Xian Sheng
中科院分区：
文献类型：
--
作者：
Cheng, Zhi Juan;Zhu, Shan Shan;Zhang, Xian Sheng
花序离体再生为花序发育的研究提供了一种简便的途径。本研究以拟南芥10期雌蕊为外植体，在含有2 mg/L玉米素和0.01mg/L吲哚乙酸的诱导培养基上获得了高频率的花序再生。在转入诱导培养基后第6天，在愈伤组织中检测到TFL 1表达，随后检测到LEAFY（LFY）表达，表明这两个基因在植物花序发育中起重要作用。为了研究干细胞组织中心的形成，我们检测了花序再生过程中愈伤组织内WUSCHEL（WUS）和CLAVATA 3（CLV 3）的表达。在诱导后第4天，WUS信号开始在愈伤组织上积累，然后，在花序诱导培养基上第5天，在愈伤组织上诱导CLV 3信号。WUS的表达域低于CLV 3的表达域，表明组织中心和干细胞形成的模式与合子和体细胞胚胎发生中的模式相似。但愈伤组织中的组织中心细胞比原胚多，表明花序分化需要更多的组织中心细胞。此外，发现WUS的表达受细胞分裂素与生长素的比例控制。结果表明，除了WUS和CLV 3外，花序再生还需要其他因素。
Inflorescence regeneration in vitro provides a simplified approach for the study of inflorescence development. In this study, high frequency of regenerated inflorescences was established using Arabidopsis stage-10 pistil as the explants on the inducing medium containing the 2 mg/L zeatin and 0.01 mg/L indole-3-acetic acid. TERMINAL FLOWER 1 (TFL1) expression was detected in callus at 6 days after transferred to inducing medium, and LEAFY (LFY) expression was detectable subsequently, suggesting that both genes play important roles as they function on inflorescence development in the plant. To investigate the formation of the stem cell organizing center, we examined the WUSCHEL (WUS) and CLAVATA3 (CLV3) expression within callus during inflorescence regeneration. WUS signals start to accumulate on callus at 4 days after induction, and then, the CLV3 signals are induced on callus at 5 days on the inflorescence-inducing medium. The expression domain of WUS is below that of CLV3, indicating that the patterns of the organizing center and stem cell formation are similar to that in zygotic and somatic embryogenesis. However, more cells of the organizing center were observed within callus than pro-embryo, suggesting that inflorescence differentiation requires more cells of the organizing center. Furthermore, it was found that the WUS expression is controlled by the ratio of cytokinin with auxin. The results suggest that other factors besides WUS and CLV3 are required for inflorescence regeneration.