Fixation compliance in a mouse osteotomy model induces two different processes of bone healing but does not lead to delayed union
Fixation compliance in a mouse osteotomy model induces two different processes of bone healing but does not lead to delayed union
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DOI：
10.1016/j.jbiomech.2009.06.004
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发表时间：
2009-09-18
影响因子：
2.4
通讯作者：
Ito, Keita
中科院分区：
文献类型：
--
作者：
Groengroeft, Ina;Heil, Petra;Ito, Keita
延迟愈合是骨折愈合的一个问题并发症，其病理生理学尚不清楚。先进的小鼠分子生物学方法将有利于研究。在人类中，固定刚度降低和复位不良通常与延迟愈合有关。本研究将这两种因素结合起来，观察它们对小鼠骨愈合的影响。使用两个带锁定螺钉的接骨板（其中一个的弯曲刚度是另一个的1/4）来稳定0.45 mm间隙截骨。mu CT、X线片、4pt弯曲试验和组织学分析表明，不同的接骨板类型导致两种不同的愈合途径。柔性较小的桥接板仅诱导膜内骨化，而柔性较大的桥接板诱导软骨内骨化和膜内骨化的混合物。然而，在术后14至21天期间，不同的钢板导致愈合延迟仅3-5天。在小鼠中，相当大的固定灵活性是诱导与人类相似的继发性骨愈合所必需的，但这不足以诱导人类预期的骨愈合显著延迟。(C)2009爱思唯尔有限公司保留所有权利。
Delayed unions are a problematic complication of fracture healing whose pathophysiology is not well understood. Advanced molecular biology methods available with mice would be advantageous for investigation. In humans, decreased fixation rigidity and poor reduction are generally associated with delayed unions. In this study, these two factors were combined to observe their effect on bone healing in mice. Two plates with locking screws, one with 1/4 the bending stiffness of the other, were used to stabilize a 0.45 mm gap osteotomy. mu CT, radiographs, 4pt-bending tests and histological analysis demonstrated that the different plate types led to two different healing pathways. The less flexible bridging plate induced only intramembranous ossification whereas the more flexible bridging plate induced a mixture of endochondral and intramembranous ossification. However, the different plates led to a delay in healing of only 3-5 days in the period between 14 and 21 post-operative days. In mice, considerable fixation flexibility is necessary to induce secondary bone healing similar to that which occurs in humans, but this was not sufficient to induce a substantial delay in bone healing as would be expected in humans. (C) 2009 Elsevier Ltd. All rights reserved.