BVES is a novel interactor of ANO5 and regulates myoblast differentiation.
BVES is a novel interactor of ANO5 and regulates myoblast differentiation.
复制标题
BVES 是 ANO5 的一种新型相互作用因子，可调节成肌细胞分化。
DOI：
10.1186/s13578-021-00735-w
复制
发表时间：
2021-12-28
影响因子：
7.5
通讯作者：
Han R
中科院分区：
文献类型：
--
作者：
Li H;Xu L;Gao Y;Zuo Y;Yang Z;Zhao L;Chen Z;Guo S;Han R
Anoctamin 5(ANO5)是一种膜蛋白，属于TMEM16/Anoctamin家族，它的缺失导致肢体带状肌营养不良R12(LGMDR12)的发生。然而，对ANO5的相互作用组及其细胞功能知之甚少。在这项研究中，我们利用近端标记方法来鉴定稳定表达BioID2标记的ANO5的C2C12成肌细胞中ANO5的相互作用蛋白。质谱仪鉴定了41个独特的蛋白质，包括BVES和POPDC3，特别是从ANO5-BioID2样品中，但不是从与ANO6或MG53融合的BioID2中。免疫共沉淀法(Co-IP)进一步证实了ANO5与BVES的相互作用，ANO5的N端介导了与BVES的C端的相互作用。ANO5和BVES共同定位于肌肉细胞，并在内质网(ER)膜上富集。基因组编辑介导的ANO5或BVES干扰显着抑制C2C12成肌细胞的分化，而对增殖几乎没有影响。综上所述，这些数据表明BVES是ANO5的一种新的相互作用蛋白，参与肌肉分化的调节。网上版载有补充材料，可在10.1186/s13578-021-00735-w查阅。
Anoctamin 5 (ANO5) is a membrane protein belonging to the TMEM16/Anoctamin family and its deficiency leads to the development of limb girdle muscular dystrophy R12 (LGMDR12). However, little has been known about the interactome of ANO5 and its cellular functions. In this study, we exploited a proximal labeling approach to identify the interacting proteins of ANO5 in C2C12 myoblasts stably expressing ANO5 tagged with BioID2. Mass spectrometry identified 41 unique proteins including BVES and POPDC3 specifically from ANO5-BioID2 samples, but not from BioID2 fused with ANO6 or MG53. The interaction between ANO5 and BVES was further confirmed by co-immunoprecipitation (Co-IP), and the N-terminus of ANO5 mediated the interaction with the C-terminus of BVES. ANO5 and BVES were co-localized in muscle cells and enriched at the endoplasmic reticulum (ER) membrane. Genome editing-mediated ANO5 or BVES disruption significantly suppressed C2C12 myoblast differentiation with little impact on proliferation. Taken together, these data suggest that BVES is a novel interacting protein of ANO5, involved in regulation of muscle differentiation. The online version contains supplementary material available at 10.1186/s13578-021-00735-w.
登录
查看更多内容
影响因子：
9
作者：
通讯作者：
--
DOI：
10.1083/jcb.202007059
发表时间：
2021-03-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Foltz SJ;Cui YY;Choo HJ;Hartzell HC
通讯作者：
Hartzell HC
影响因子：
64.8
作者：
Komor AC;Kim YB;Packer MS;Zuris JA;Liu DR
通讯作者：
Liu DR
影响因子：
4.8
作者：
Knight, RF;Bader, DM;Backstrom, JR
通讯作者：
Backstrom, JR
影响因子：
4
作者：
Kim, Hyungsup;Kim, Hyesu;Oh, Uhtaek
通讯作者：
Oh, Uhtaek