Acute changes in sputum IL-10 following underground exposure to diesel exhaust
Acute changes in sputum IL-10 following underground exposure to diesel exhaust
复制标题
DOI：
10.1080/15563650601072142
复制
发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Miller, Hugh B.
中科院分区：
文献类型：
--
作者：
Burgess, Jefferey L.;Fleming, Joy E.;Miller, Hugh B.
介绍。尽管暴露于柴油机尾气会对健康产生不利影响，但人们对地下工作场所暴露于柴油机尾气的严重影响知之甚少。方法。在所有地下铜矿中，使用柴油动力和气动设备，在不同的日子对与碎石清除（出渣）作业相关的 12 名受试者完成了交叉班次肺活量测定和痰液诱导，并在基线非暴露日收集了痰液。结果。对于柴油机操作，在 1-2 小时操作期间，元素碳暴露平均为 538 +/- 512 mu g/m(3)。使用一种 ELISA 测定时，痰液白细胞介素 10 随柴油机尾气而减少（3.69 vs. 2.32 pg/ml，p = 0.015），但当使用不同的 ELISA 试剂盒测量时则增加（0.18 vs. 0.59 pg/ml，p = 0.019），这与 IL-10 蛋白浓度总体下降但生物活性形式增加一致。痰中白细胞介素 6 随暴露于柴油机尾气中而降低，尽管这种变化在仅限于非吸烟者时失去了统计学意义。肺活量测定、白细胞介素1β、4和8、肿瘤坏死因子α或8-羟基-2'-脱氧鸟苷没有显着变化。结论。高水平的柴油机尾气会导致痰液 IL-10 快速变化，表明可能存在蛋白质修饰。
Introduction. Although exposure to diesel exhaust has been linked with adverse health effects, little is known about the acute effects of exposure in the underground workplace. Methods. Cross-shift spirometry and sputum induction were completed on twelve subjects associated with comminuted rock removal (mucking) operations in all underground copper mine using diesel powered and pneumatic equipment on separate days, and sputum collected on a baseline non-exposure day as well. Results. For diesel operations, elemental carbon exposure averaged 538 +/- 512 mu g/m(3) during the 1-2 hour operations. Sputum interleukin-10 decreased with diesel exhaust using one ELISA assay (3.69 v. 2.32 pg/ml, p = 0.015), but increased when measured with a different ELISA kit (0.18 v. 0.59 pg/ml, p = 0.019), consistent with an overall decline in IL-10 protein concentration but an increase in the biologically active form. Sputum interleukin-6 decreased with exposure to diesel exhaust, although this change lost statistical significance when restricted to non-smokers. There were no significant changes in spirometry, interleukins 1 beta, 4, and 8, tumor necrosis factor alpha or 8-hydroxy-2'-deoxyguanosine. Conclusion. High levels of diesel exhaust can result in rapid changes in Sputum IL-10, suggesting possible protein modification.