Determination of Water Content in Dehydrated Mammalian Cells Using Terahertz Pulsed Imaging: A Feasibility Study
Determination of Water Content in Dehydrated Mammalian Cells Using Terahertz Pulsed Imaging: A Feasibility Study
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DOI：
10.2174/1389201017666151029105941
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发表时间：
2016-01-01
影响因子：
2.8
通讯作者：
Tunnacliffe, Alan
中科院分区：
文献类型：
--
作者：
Chau, David Y. S.;Dennis, Anthony R.;Tunnacliffe, Alan
哺乳动物细胞涉及一系列生物技术应用，并且最近在再生医学中得到越来越多的利用。哺乳动物细胞成功应用的关键是其长期储存和运输，其中液氮冷冻保存是最常用的策略。然而，低温保存存在成本高、运输物流困难以及使用不良添加剂（例如动物血清或 DMSO）的问题。另一种替代方法是对哺乳动物细胞进行可行的干燥，该方法成本低、维护成本低且工艺兼容。一些研究小组声称已经实现了这一目标，但相关细胞样品的干燥程度并不总是清楚，部分原因是难以确定极低的含水量。尽管存在多种经常用于定量样品中水含量的技术（例如 FTIR 光谱、热重分析 (TGA)、NMR 光谱），但样品制备的复杂性以及所涉及的成本和时间限制是不利的。在这里，我们评估了一种新颖、快速且低成本的技术，即太赫兹（THz）光谱，用于量化脱水哺乳动物细胞样品中的水含量。
Mammalian cells are involved in a range of biotechnological applications and more recently have been increasingly exploited in regenerative medicine. Critical to successful applications involving mammalian cells are their long-term storage and transport, for which cryopreservation in liquid nitrogen is the most frequently used strategy. However, cryopreservation suffers from high costs, difficulties in transport logistics and the use of undesirable additives (e.g. animal sera or DMSO). An alternative approach, proposed as low cost, low maintenance and process-compatible, is viable desiccation of mammalian cells. Several groups claim to have achieved this, but the extent of desiccation in the cell samples concerned is not always clear, in part because of difficulties in determining very low water content. Although several techniques exist that are frequently used to quantify the amount of water in samples (e.g. FTIR spectroscopy, thermogravimetric analysis (TGA), NMR spectroscopy), the complexity of sample preparation, as well as the costs and time constraints involved are disadvantageous. Here, we assess a novel, rapid and low cost technique, i.e. terahertz (THz) spectroscopy, for the quantification of water content within dehydrated mammalian cell samples.