Transformation process of ice crystallized from a glassy dilute trehalose aqueous solution
Transformation process of ice crystallized from a glassy dilute trehalose aqueous solution
复制标题
玻璃状稀海藻糖水溶液结晶冰的转化过程
DOI：
10.1039/d2cp02712g
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发表时间：
2022
影响因子：
3.3
通讯作者：
Takeya Satoshi
中科院分区：
文献类型：
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作者：
Suzuki Yoshiharu;Takeya Satoshi
亚稳态形式的冰及其晶体生长在气象学、低温生物学和行星科学中起着重要作用。然而，由于溶质的偏析，研究溶质对冰在稀水溶液中晶体生长的影响是困难的。在此，我们制作了稀海藻糖水溶液（0.023摩尔分数）的非隔离玻璃，并使用粉末X射线衍射测量来检查水溶液中结晶冰随着温度升高的转化。在结晶后立即形成的冰是具有很少堆垛层错的纳米尺寸的堆垛无序冰（冰Isd），并且具有高立方度。海藻糖水溶液中的冰Isd的晶体生长明显慢于甘油水溶液中的冰Isd和纯冰Isd。冰Isd的存活温度高达230 K，高于纯水从冰Isd到六方冰（冰Ih）的转变温度（200 K）。海藻糖的存在抑制了冰Isd的晶体生长，因此，冰在真空条件下容易升华。此外，宏观偏析的发生在245 K的Isd到Ih的转变有关。这些结果对于改进冻存生物组织的解冻技术和理解地球大气中冰云形成的机制具有重要意义。
Metastable forms of ice and their crystal growth play an important role in meteorology, cryobiology, and planetary science. However, it is difficult to investigate the effects of solute on the crystal growth of ice in a dilute aqueous solution due to the segregation. Herein, we made a non-segregated glass of dilute trehalose aqueous solution (0.023 mole fraction) and examined the transformation of crystalline ice in the aqueous solution with increasing temperature using powder X-ray diffraction measurements. The ice formed immediately after the crystallization is nano-sized stacking disordered ice (ice Isd) with few stacking faults and has high cubicity. The crystal growth of ice Isd in the trehalose aqueous solution was remarkably slower than those of ice Isd in a glycerol aqueous solution and pure ice Isd. The ice Isd survived up to ∼230 K which is higher than the transformation temperature from ice Isd to hexagonal ice (ice Ih) of pure water (∼200 K). The existence of trehalose inhibits the crystal growth of ice Isd and, as a result, the ice sublimates easily under vacuum conditions. Moreover, the occurrence of macroscopic segregation at ∼245 K is related to the Isd-to-Ih transformation. These results are important for the improvement of thawing techniques for cryopreserved biological tissues and for the understanding of the mechanism of ice cloud formation in the Earth's atmosphere.