Antisense expression of the CK2 alpha-subunit gene in Arabidopsis. Effects on light-regulated gene expression and plant growth.
Antisense expression of the CK2 alpha-subunit gene in Arabidopsis. Effects on light-regulated gene expression and plant growth.
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发表时间：
1999
期刊：
影响因子：
7.4
通讯作者：
Y. Lee;A. Lloyd;S. Roux
中科院分区：
文献类型：
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作者：
Y. Lee;A. Lloyd;S. Roux
蛋白激酶CK2(以前的酪蛋白激酶II)被认为参与了植物光调控基因的表达，因为它在体外能够磷酸化转录因子，这些转录因子结合在光调控基因的启动子区域。为了在体内解决这种可能性，并更多地了解CK2在植物中的潜在生理作用，我们将CK2α亚单位基因的反义构建转化拟南芥，并研究了形态和分子表型。反义转化子具有较小的成虫叶片大小，在黑暗中Chs的表达增加，红光处理后CaB和RBC的表达增加。后者的分子表型表明，CK2可能是光调控基因表达的几个负的数量效应因子之一。对CK2的作用机制及其参与光敏色素信号转导途径进行了讨论。
The protein kinase CK2 (formerly casein kinase II) is thought to be involved in light-regulated gene expression in plants because of its ability to phosphorylate transcription factors that bind to the promoter regions of light-regulated genes in vitro. To address this possibility in vivo and to learn more about the potential physiological roles of CK2 in plants, we transformed Arabidopsis with an antisense construct of the CK2 alpha-subunit gene and investigated both morphological and molecular phenotypes. Antisense transformants had a smaller adult leaf size and showed increased expression of chs in darkness and of cab and rbcS after red-light treatment. The latter molecular phenotype implied that CK2 might serve as one of several negative and quantitative effectors in light-regulated gene expression. The possible mechanism of CK2 action and its involvement in the phytochrome signal transduction pathway are discussed.