E-cadherin is required for gastrulation cell movements in zebrafish
E-cadherin is required for gastrulation cell movements in zebrafish
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DOI：
10.1016/j.mod.2005.03.008
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发表时间：
2005-06-01
影响因子：
2.6
通讯作者：
Hibi, M
中科院分区：
文献类型：
--
作者：
Shimizu, T;Yabe, T;Hibi, M
E-钙粘蛋白是经典钙粘蛋白家族的成员，并且已知参与细胞-细胞粘附和多种组织的粘附依赖性形态发生。我们分离出一个斑马鱼突变体（cdh 1（rk 3）），它在e-钙粘蛋白/cdh 1基因中有一个突变。突变rk 3是一个亚纯型等位基因，纯合突变胚胎在原肠胚形成和组织形态发生中表现出不同的表型。受影响最严重的胚胎表现出外延延迟，收敛和伸展运动减少，细胞从胚胎中分离，导致早期胚胎死亡。受影响不太严重的胚胎存活通过咽阶段，并表现出扁平的前神经组织，异常的定位和形态的孵化腺，分散的三叉神经节，和三叉神经节的异常轴突束。母合子cdh 1（rk 3）胚胎在原肠胚形成过程中表现为外生停滞，其中被膜层（EVL）和卵黄合胞体层完成外生，而深层细胞（DC）未完成外生。在胚胎中观察到类似的表型，接受反义吗啉代寡核苷酸（cdh 1 MO）对E-钙粘蛋白，并在斑马鱼epiboly突变体。与斑马鱼epiboly突变体weg的互补分析表明cdh 1（rk 3）与half bakedlweg是等位基因。免疫组织化学与抗β-连环蛋白抗体和电子显微镜显示，DC和EVL之间的粘附大多被破坏，但DC之间的粘附相对不受影响的MZcdh 1（rk 3）突变体和cdh 1变形胚胎。这些数据表明，E-钙粘蛋白介导的DC和EVL之间的细胞粘附在斑马鱼的外皮运动中发挥作用。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
E-cadherin is a member of the classical cadherin family and is known to be involved in cell-cell adhesion and the adhesion-dependent morphogenesis of various tissues. We isolated a zebrafish mutant (cdh1(rk3)) that has a mutation in the e-cadherin/cdh1 gene. The mutation rk3 is a hypomorphic allele, and the homozygous mutant embryos displayed variable phenotypes in gastrulation and tissue morphogenesis. The most severely affected embryos displayed epiboly delay, decreased convergence and extension movements, and the dissociation of cells from the embryos, resulting in early embryonic lethality. The less severely affected embryos survived through the pharyngula stage and showed flattened anterior neural tissue, abnormal positioning and morphology of the hatching gland, scattered trigeminal ganglia, and aberrant axon bundles from the trigeminal ganglia. Matemal-zygotic cdh1(rk3) embryos displayed epiboly arrest during gastrulation, in which the enveloping layer (EVL) and the yolk syncytial layer but not the deep cells (DC) completed epiboly. A similar phenotype was observed in embryos that received antisense morpholino oligonucleotides (cdh1MO) against E-cadherin, and in zebrafish epiboly mutants. Complementation analysis with the zebrafish epiboly mutant weg suggested that cdh1(rk3) is allelic to half bakedlweg. Immunohistochemistry with an anti-beta-catenin antibody and electron microscopy revealed that adhesion between the DCs and the EVL was mostly disrupted but the adhesion between DCs was relatively unaffected in the MZcdh1(rk3) mutant and cdh1 morphant embryos. These data suggest that E-cadherin-mediated cell adhesion between the DC and EVL plays a role in the epiboly movement in zebrafish. (c) 2005 Elsevier Ireland Ltd. All rights reserved.