Transformation of Schizosaccharomyces japonicus.
Transformation of Schizosaccharomyces japonicus.
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DOI：
10.1101/pdb.prot091850
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发表时间：
2017-12-01
影响因子：
--
通讯作者：
Niki, Hironori
中科院分区：
文献类型：
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作者：
Aoki, Keita;Niki, Hironori
该方案描述了使用电穿孔来用质粒或线性DNA转化裂殖酵母。质粒有助于突变的互补检测和特定基因的表达。通过同源重组整合到染色体DNA中的线性DNA片段可用于基因缺失或将基因与标签序列（例如，编码荧光蛋白）。将DNA导入S.因此，推荐电穿孔法，因为S.醋酸锂（LiAc）是醋酸锂的敏感物质。
This protocol describes the use of electroporation to transform Schizosaccharomyces japonicus with plasmids or linear DNA. Plasmids are helpful for the complementation testing of mutations and for the expression of specific genes. Linear DNA fragments integrated into chromosomal DNA by homologous recombination are useful for gene deletion or to fuse a gene with a tag sequence (e.g., encoding a fluorescent protein). To introduce DNA into S. japonicus, electroporation methods are recommended because S. japonicus is sensitive to lithium acetate (LiOAc).