Structure and nucleotide sequence of the rat intestinal vitamin D-dependent calcium binding protein gene.
Structure and nucleotide sequence of the rat intestinal vitamin D-dependent calcium binding protein gene.
复制标题
大鼠肠道维生素 D 依赖性钙结合蛋白基因的结构和核苷酸序列。
DOI：
10.1073/pnas.85.23.8988
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发表时间：
1988
影响因子：
11.1
通讯作者：
DeLuca,HF
中科院分区：
文献类型：
--
作者：
Krisinger,J;Darwish,H;Maeda,N;DeLuca,HF
维生素 D 依赖性肠钙结合蛋白 (ICaBP，9 kDa) 受到 1,25-二羟基维生素 D3 [1,25-(OH)2D3]（维生素的激素活性形式）的转录调节。为了研究1,25-(OH)2D3的基因调控机制，我们使用cDNA探针分离了大鼠ICaBP基因。其核苷酸序列显示 3 个外显子被 2 个内含子分隔，长度约为 3 KB。第一个外显子仅代表非编码序列，而第二个和第三个外显子编码蛋白质的两个钙结合结构域。该基因在第一个内含子中包含一个 15 个碱基对的不完美回文，与雌激素反应元件具有高度同源性。该序列可能代表参与 ICaBP 基因调节的维生素 D 响应元件。第二个内含子显示一个 84 个碱基对长的简单核苷酸重复，暗示 Z-DNA 的形成。基因组 Southern 分析表明，大鼠基因为单个拷贝。
The vitamin D-dependent intestinal calcium binding protein (ICaBP, 9 kDa) is under transcriptional regulation by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], the hormonal active form of the vitamin. To study the mechanism of gene regulation by 1,25-(OH)2D3, we isolated the rat ICaBP gene by using a cDNA probe. Its nucleotide sequence revealed 3 exons separated by 2 introns within approximately 3 kilobases. The first exon represents only noncoding sequences, while the second and third encode the two calcium binding domains of the protein. The gene contains a 15-base-pair imperfect palindrome in the first intron that shows high homology to the estrogen-responsive element. This sequence may represent the vitamin D-responsive element involved in the regulation of the ICaBP gene. The second intron shows an 84-base-pair-long simple nucleotide repeat that implicates Z-DNA formation. Genomic Southern analysis shows that the rat gene is represented as a single copy.