Protocol to generate human PSC-derived pancreatic beta-cells through methionine and zinc deprivation
Protocol to generate human PSC-derived pancreatic beta-cells through methionine and zinc deprivation
复制标题
通过蛋氨酸和锌剥夺生成人 PSC 来源的胰腺 β 细胞的方案
DOI：
10.1016/j.xpro.2023.102183
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Kume S
中科院分区：
文献类型：
--
作者：
Sim Z.E;Enomoto T;Shiraki N;Kume S
人们已努力建立模拟胰腺发育的分化方案并衍生用于再生医学的胰腺β细胞。在这里，我们提出了一种使用人类多能干细胞优化的胰腺 β 细胞分化程序。我们描述了短暂的 5 小时甲硫氨酸剥夺预处理的步骤，然后在定形内胚层分化阶段应用缺锌培养基以提高分化效率。蛋氨酸和锌剥夺的应用促进了功能性胰腺 β 细胞的生成。有关该协议的使用和执行的完整详细信息，请参阅 Sim 等人。 (2022).1
Efforts have been made to establish a differentiation protocol mimicking pancreatic development and to derive pancreatic β cells for regenerative medicine. Here, we present an optimized pancreatic β cell differentiation procedure using human pluripotent stem cells. We describe steps for a short 5-h methionine deprivation pretreatment followed by the application of zinc-deprived media at definitive endoderm differentiation stages to improve differentiation efficiency. The application of methionine and zinc deprivation facilitates the generation of functional pancreatic β cells.For complete details on the use and execution of this protocol, please refer to Sim et al. (2022).1