Characterization of protein films on dental materials: Bicinchoninic acid assay (BCA) studies on loosely and firmly adsorbed protein layers
Characterization of protein films on dental materials: Bicinchoninic acid assay (BCA) studies on loosely and firmly adsorbed protein layers
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DOI：
10.1002/pssa.201200642
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发表时间：
2013-05
期刊：
影响因子：
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通讯作者：
F. Kratz;C. Müller;N. Körber;N. Umanskaya;M. Hannig;C. Ziegler
中科院分区：
文献类型：
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作者：
F. Kratz;C. Müller;N. Körber;N. Umanskaya;M. Hannig;C. Ziegler
用比胆酸测定法(BCA法)研究了BSA在牙科钛和牙科金表面的松散和牢固吸附情况。这种方法不需要样品的特殊性质，如高反射率或导电性，使BCA分析成为研究不同底物材料(如天然釉质)的广泛光谱的宝贵工具。这项工作中的实验表明，使用BCA分析可以区分松散结合和牢固结合的蛋白质。在所研究的牙科钛和牙科金样上，BSA的吸附量主要以松散结合部分为主。牙科金表面BSA的吸附总量显着高于钛表面。在牙科钛和牙科金的等电点附近，松散结合的BSA含量较高，而牙科钛上牢固吸附的BSA的数量则遵循静电的规律。在高pH值(pH 9.0)下，BSA分子的构象变化在吸附过程中起关键作用。
The loosely and firmly adsorbed amount of bovine serum albumin (BSA) on dental titanium and dental gold was investigated by bicinchoninic acid assay (BCA assay). This method does not require special properties of the sample like a high reflectivity or conductivity and turns the BCA assay into a valuable tool to investigate a widespread spectrum of different substrate materials, e.g., natural enamel. The experiments in this work showed that it is possible to distinguish between loosely and firmly bound protein with the BCA assay. On the here investigated dental titanium and dental gold samples the amount of adsorbed BSA was dominated by the loosely bound part. On dental gold the total amount of adsorbed BSA was significantly higher than on titanium. The amount of loosely bound BSA was high near the isoelectric points of dental titanium and dental gold while the amount of firmly adsorbed BSA on dental titanium followed the electrostatics. At high pH values (pH 9.0) conformational changes of the BSA molecules played a key role in the adsorption process.