Genetic assignment tests reveal dispersal of white-tailed deer: implications for chronic wasting disease
Genetic assignment tests reveal dispersal of white-tailed deer: implications for chronic wasting disease
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DOI：
10.1644/13-mamm-a-167
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发表时间：
2014-06-26
影响因子：
1.7
通讯作者：
Novakofski, Jan
中科院分区：
文献类型：
--
作者：
Green, Michelle L.;Manjerovic, Mary Beth;Novakofski, Jan
调查新病例的感染源对于有效的疾病管理至关重要。2002年，在伊利诺伊州的白尾鹿（Odocoileus virginianus）中首次发现了慢性消耗性疾病（CWD）。虽然慢性消耗病集中在北方伊利诺斯州，但2011年从疾病集中地以南100公里以上的地点取样了4只受感染的鹿。我们使用分配测试（GENECLASS 2和ONCOR）来确定感染鹿的可能遗传源位置。我们的基线数据集包括从10个地点采样的310只鹿。从基线数据集，我们确定了15个CWD阳性和15个CWD阴性鹿的最可能的遗传源位置。共有17-20%的动物返回分配到其样品位置作为其最可能的遗传源位置，其余动物交叉分配到另一个位置。GENECLASS 2的地点之间平均距离为41.4公里，ONCOR为43.4公里（范围0.0-90.8公里）。对于阳性和阴性动物，来源和采样位置之间的距离相似。使用ONCOR，雄性动物的距离大于雌性动物的距离，但基于年龄的距离无差异。由于白尾鹿的基因流动几乎没有障碍，管理人员应该减少在CWD感染地区的鹿的运动，以减少CWD的直接和间接传播。
Investigating sources of infection for new disease cases is critical to effective disease management. Chronic wasting disease (CWD) was first detected among white-tailed deer (Odocoileus virginianus) in Illinois in 2002. Although CWD was focused in northern Illinois, 4 infected deer were sampled in 2011 from locations greater than 100 km south of the disease focus. We used assignment tests (GENECLASS2 and ONCOR) to determine a likely genetic source location for infected deer. Our baseline data set consisted of 310 deer sampled from 10 locations. From the baseline data set, we determined the most likely genetic source location of 15 CWD-positive and 15 CWD-negative deer. A total of 17-20% back-assigned to their sample location as their most likely genetic source location and the remainder of the animals cross-assigned to another location. The average distance between locations was 41.4 km for GENECLASS2 and 43.4 km for ONCOR (range 0.0-90.8 km). Distances between source and sampling locations were similar for positive and negative animals Distances for males were greater than those for females using ONCOR, but there was no difference in distance based on age. Because there are few barriers to gene flow for white-tailed deer, managers should reduce movement of deer in CWD-infected areas in an effort to reduce direct and indirect transmission of CWD.