The Coiled-Coil and Nucleotide Binding Domains of BROWN PLANTHOPPER RESISTANCE14 Function in Signaling and Resistance against Planthopper in Rice
The Coiled-Coil and Nucleotide Binding Domains of BROWN PLANTHOPPER RESISTANCE14 Function in Signaling and Resistance against Planthopper in Rice
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褐飞虱抗性 14 的卷曲螺旋和核苷酸结合域在水稻抗稻飞虱信号传导和抗性中的作用
DOI：
10.1105/tpc.17.00263
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发表时间：
2017-12-01
期刊：
影响因子：
11.6
通讯作者：
He, Guangcun
中科院分区：
文献类型：
--
作者：
Hu, Liang;Wu, Yan;He, Guangcun
褐飞虱抗性基因14（BPH14）是第一个通过图位克隆获得的水稻抗稻飞虱基因，编码一个卷曲螺旋、核苷酸结合位点、富含亮氨酸重复序列（CC-NB-LRR）蛋白。最近已经鉴定了几个编码具有相似结构的蛋白质的抗稻飞虱和蚜虫基因。在这里，我们分析了BPH14的结构域的功能，以确定BPH14介导的抗稻飞虱的分子机制。单独或组合的CC或NB结构域（CC-NB [CN]）赋予与全长（FL）BPH 14相似水平的褐飞虱抗性。这两个结构域激活水杨酸信号通路和防御基因的表达。在水稻原生质体和烟草叶片中，这些结构域增加了活性氧水平，而不触发细胞死亡。此外，抗性结构域和FL BPH14蛋白形成与转录因子WRKY46和WRKY72相互作用的同源复合物。在水稻原生质体中，FL BPH14或其CC、NB和CN结构域的表达增加了WRKY46和WRKY72的积累以及WRKY46和WRKY72依赖的反式激活活性。WRKY46和WRKY72与受体样细胞质激酶基因RLCK 281和愈伤组织糖合成酶基因LOC_Os01g67364.1的启动子结合，其反式激活活性依赖于WRKY46或WRKY72。这些发现揭示了这一重要的昆虫抗性机制。
BROWN PLANTHOPPER RESISTANCE14 (BPH14), the first planthopper resistance gene isolated via map-based cloning in rice (Oryza sativa), encodes a coiled-coil, nucleotide binding site, leucine-rich repeat (CC-NB-LRR) protein. Several planthopper and aphid resistance genes encoding proteins with similar structures have recently been identified. Here, we analyzed the functions of the domains of BPH14 to identify molecular mechanisms underpinning BPH14-mediated planthopper resistance. The CC or NB domains alone or in combination (CC-NB [CN]) conferred a similar level of brown planthopper resistance to that of full-length (FL) BPH14. Both domains activated the salicylic acid signaling pathway and defense gene expression. In rice protoplasts and Nicotiana benthamiana leaves, these domains increased reactive oxygen species levels without triggering cell death. Additionally, the resistance domains and FL BPH14 protein formed homocomplexes that interacted with transcription factors WRKY46 and WRKY72. In rice protoplasts, the expression of FL BPH14 or its CC, NB, and CN domains increased the accumulation of WRKY46 and WRKY72 as well as WRKY46-and WRKY72-dependent transactivation activity. WRKY46 and WRKY72 bind to the promoters of the receptor-like cytoplasmic kinase gene RLCK281 and the callose synthase gene LOC_Os01g67364.1, whose transactivation activity is dependent on WRKY46 or WRKY72. These findings shed light on this important insect resistance mechanism.