A sequence-anchored genetic linkage map for the moss, Physcomitrella patens.
A sequence-anchored genetic linkage map for the moss, Physcomitrella patens.
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DOI：
10.1111/j.1365-313x.2008.03637.x
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发表时间：
2008-12
期刊：
影响因子：
--
通讯作者：
Cuming AC
中科院分区：
文献类型：
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作者：
Kamisugi Y;von Stackelberg M;Lang D;Care M;Reski R;Rensing SA;Cuming AC
藓类小立碗藓是植物细胞生物学研究的模式植物，由于其在陆地植物发生学中的基础地位，可用于比较分析植物基因功能和发育的进化。由于其通过同源重组进行靶向转基因整合的出色能力，它非常适合于“反向遗传”分析。然而，由于缺乏遗传连锁图谱，通过诱变和图位克隆进行基因鉴定迄今为止是不可能的。利用分子标记[扩增片段长度多态性（AFLP）和简单重复序列（SSR）]，我们已经产生了立碗藓的遗传连锁图谱。在46个连锁群中定位了179个基因特异性SSR标记，共鉴定出1574个多态性AFLP标记。整合SSR和AFLP图谱产生31个连锁群，包括1420个标记。锚定的基因特异性SSR标记加上AFLP位点的计算预测的综合连锁图，使其对应于新测序的立碗藓基因组。产生的连锁图密集填充分子标记和锚定的基因组序列，现在提供了一个资源，向前遗传询问的有机体和管道的发展，基于地图克隆立碗藓基因。这将从根本上提高立碗藓的潜力，以确定基因功能如何演变为收购复杂的发展战略，在植物王国。
The moss Physcomitrella patens is a model for the study of plant cell biology and, by virtue of its basal position in land plant phylogeny, for comparative analysis of the evolution of plant gene function and development. It is ideally suited for ‘reverse genetic’ analysis by virtue of its outstanding ability to undertake targeted transgene integration by homologous recombination. However, gene identification through mutagenesis and map-based cloning has hitherto not been possible, due to the lack of a genetic linkage map. Using molecular markers [amplified fragment length polymorphisms (AFLP) and simple sequence repeats (SSR)] we have generated genetic linkage maps for Physcomitrella. One hundred and seventy-nine gene-specific SSR markers were mapped in 46 linkage groups, and 1574 polymorphic AFLP markers were identified. Integrating the SSR- and AFLP-based maps generated 31 linkage groups comprising 1420 markers. Anchorage of the integrated linkage map with gene-specific SSR markers coupled with computational prediction of AFLP loci has enabled its correspondence with the newly sequenced Physcomitrella genome. The generation of a linkage map densely populated with molecular markers and anchored to the genome sequence now provides a resource for forward genetic interrogation of the organism and for the development of a pipeline for the map-based cloning of Physcomitrella genes. This will radically enhance the potential of Physcomitrella for determining how gene function has evolved for the acquisition of complex developmental strategies within the plant kingdom.