Human platelets can activate peripheral blood B cells and increase production of immunoglobulins
Human platelets can activate peripheral blood B cells and increase production of immunoglobulins
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DOI：
10.1016/j.exphem.2007.05.021
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发表时间：
2007-09-01
影响因子：
2.6
通讯作者：
Garraud, Olivier
中科院分区：
文献类型：
--
作者：
Cognasse, Fabrice;Hameh-Cognasse, Hind;Garraud, Olivier
目标。血小板代表着止血、炎症和组织修复之间的联系。它们在免疫反应和炎症中的作用主要涉及许多分子，其中包括toll样受体、主要组织相容性复合体I类、CD40和CD154/CD40配体（CD40L）。由于血小板是可溶性CD40L （sCD40L）的主要供应者，我们试图确定它们在CD40/CD40L依赖性免疫反应中的作用，并了解血小板和外周B淋巴细胞之间的相互作用。材料与方法。我们通过流式细胞术和共聚焦扫描激光显微镜检查了血小板结合非刺激B细胞的能力和表型变化。细胞因子/趋化因子的调节；用夹心酶联免疫吸附法测定共培养、血小板和B淋巴细胞上清液中免疫球蛋白（Ig） A、IgG、IgM和IgG亚类的总水平，用逆转录酶聚合酶链反应测定细胞因子mRNA的差异产生。在共培养中，血小板和B淋巴细胞相互激活，血小板CD62p和B细胞CD86的表达增加。血小板/B细胞相互作用伴随着血小板和B淋巴细胞CD40和CD40L膜表达的变化。IL12p70和IL8基因转录明显降低，这与B细胞有关。相反，sCD40L和RANTES mRNA的表达有显著的血小板依赖性降低。与血小板孵育3天后，分化的B细胞增加了体外产生IgG1、IgG2和IgG3，但不增加IgG4、IgA或igm。这些数据强调了血小板在适应性免疫反应中的潜在重要作用。血小板具有免疫调节作用，可能应用于临床多次输血患者（如造血干细胞移植）。(c) 2007年ISEH血液学和干细胞学会。Elsevier Inc.出版。
Objective. Blood platelets represent a link between hemostasis, inflammation, and tissue repair. Their role in immune responses and inflammation mainly involves many molecules, among which Toll-like receptor, major histocompatibility complex class I, CD40 and CD154/CD40 ligand (CD40L). As platelets are the major purveyor of soluble CD40L (sCD40L), we sought to determine their involvement in CD40/CD40L-dependent immune responses and to understand the interactions between platelets and peripheral B lymphocytes.Materials and Methods. We examined the capacity of platelets to bind nonstimulated B cells, and phenotypic changes by flow cytometry and confocal scanning laser microscopy. Modulation of cytokines/chemokines; and total levels of immunoglobulin (Ig) A, IgG, IgM, and IgG subclasses in supernatants of coculture, platelets, and B lymphocytes was performed by sandwich enzyme-linked immunosorbent assay and differential production of cytokine mRNA as determined by reverse transcriptase polymerase chain reaction.Results. In coculture, platelets and B lymphocytes were mutually activated, as demonstrated by the increased expression of platelet CD62p and B-cell CD86. Platelet/B-cell interactions were accompanied by changes in membrane expression of CD40 and CD40L by both platelets and B lymphocytes. IL12p70 and IL8 gene transcription were significantly reduced, which was attributable to B cells. Conversely, there was a significant, platelet-dependent reduction of sCD40L and RANTES mRNA expression. After a 3-day incubation with platelets, differentiated B cells increased their in vitro production of IgG1, IgG2, and IgG3, but not IgG4, IgA, or IgM.Conclusion. These data emphasize the potentially important role of platelets in the adaptive immune response. Platelets have an immunoregulatory role that might be applied clinically in multitransfused patients (e.g., hematopoietic stem cell transplantation). (c) 2007 ISEH Society for Hematology and Stem Cells. Published by Elsevier Inc.