Production of interferon-γ by microglia
Production of interferon-γ by microglia
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DOI：
10.1177/1352458506070763
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发表时间：
2006-10-01
影响因子：
5.8
通讯作者：
Suzumura, A.
中科院分区：
文献类型：
--
作者：
Kawanokuchi, J.;Mizuno, T.;Suzumura, A.
神经细胞通常不与免疫细胞相互作用，因为缺乏主要组织相容性复合体（MHC）抗原表达。干扰素-γ（IFN-γ）通过诱导神经细胞中的MHC抗原表达来实现这种相互作用。因此，IFN-γ是中枢神经系统（CNS）病理发展的关键细胞因子。然而，IFN-γ被认为仅由淋巴样细胞产生。在这里，我们首次表明，小鼠小胶质细胞产生IFN-γ响应IL-12和/或IL-18，使用RT-PCR检测IFN-γ，γ mRNA和免疫印迹和免疫组织化学分析IFN-γ的胞浆表达。用IL-12和IL-18刺激小胶质细胞导致小胶质细胞中MHC 11类mRNA的表达。由于IL-12和IL-18在CNS中由神经胶质细胞产生，这些细胞因子可在神经-免疫细胞相互作用的起始和通过诱导IFN-γ和MHC抗原诱导CNS中的自身免疫过程中起关键作用。
Neural cells do not usually interact with immune cells because of the lack of major histocompatibility complex (MHC) antigen expression. Interferon-gamma (IFN-,gamma) enables this interaction via induction of MHC antigen expression in neural cells. Thus, IFN-gamma is a critical cytokine for the development of central nervous system (CNS) pathologies. IFN-,gamma, however, is considered to be produced exclusively by lymphoid cells. Here, we show for the first time that murine microglia produce IFN-gamma in response to IL-12 and/or IL-18, using RT-PCR detection of IFN-,gamma mRNA and Western blotting and immunohistochemical analysis for cytoplasmic expression of IFN-gamma. Stimulation of microglia with IL-12 and IL-18 resulted in MHC class 11 mRNA expression in microglia. Since IL-12 and IL-18 are produced in the CNS by glial cells, these cytokines may play a critical role in the initiation of neural-immune cell interaction and the induction of autoimmune processes in the CNS via induction of IFN-gamma and MHC antigens.