In vitro antitumor activity of Gracilaria corticata (a red alga) against Jurkat and molt-4 human cancer cell lines
In vitro antitumor activity of Gracilaria corticata (a red alga) against Jurkat and molt-4 human cancer cell lines
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DOI：
10.5897/ajb10.602
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发表时间：
2010-10
影响因子：
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通讯作者：
K. Zandi;S. Tajbakhsh;I. Nabipour;Z. Rastian;F. Yousefi;S. Sharafian
中科院分区：
文献类型：
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作者：
K. Zandi;S. Tajbakhsh;I. Nabipour;Z. Rastian;F. Yousefi;S. Sharafian
江蓠是一种红藻，可在世界各地的许多海岸采集，如中国、印度、波斯湾等。波斯湾是一个独特的海洋栖息地，生长着多种海藻。本研究的目的是探索从布什尔海岸（伊朗西南部）采集的 G. corticata 粗提物的抗癌潜力。在这里，测试了不同浓度的 G. corticata 水提取物对 Jurkat 和 molt4 人淋巴细胞白血病细胞系可能的抗肿瘤活性。用不同浓度的藻提取物处理细胞，并用台盼蓝测定活细胞数。此外，通过甲基噻唑基四唑（MTT）测定评估提取物的细胞毒性。结果表明，9.336 µg/µl 和 9.726 µg/µl 的藻提取物分别是针对 Jurkat 和 molt-4 细胞的最有效浓度。红藻 G. corticata 的水粗提取物具有显着的抗癌活性，它可能是进一步研究的良好候选者，以开发作为抗癌剂的天然化合物，可用于生产潜在的抗癌药物和新型药物先导物。
Gracilaria corticata is a red alga which can be collected from many sea coasts around the world such as China, India, Persian Gulf, etc. The Persian Gulf is a unique marine habitat infested with diverse seaweeds. The aim of the present study is to explore anticancer potential of the crude extracts from G. corticata which was collected from the Bushehr coast (South west of Iran). Here, different concentration of the aqueous extract from G. corticata was tested for probable antitumoral activity on Jurkat and molt4 human lymphoblastic leukemic cell lines. The cells were treated by different concentration of algal extract and the number of viable cells was determined by trypan blue. Also, cytotoxicity of the extract was evaluated by methyl thiazolyl tetrazolium (MTT) assay. The results showed that 9.336 and 9.726 µg/µl of algal extract were the most effective concentrations against Jurkat and molt-4 cells, respectively. The water crude extract of red alga G. corticata had significant anticancer activity and it might be a good candidate for further investigations in order to develop a natural compound as an anticancer agent which can be used for the production of potential anticancer drug and novel pharmaceutical leads.