Optimization and kinetic modelling of robinetin and dihydrorobinetin extraction from Robinia pseudoacacia wood
Optimization and kinetic modelling of robinetin and dihydrorobinetin extraction from Robinia pseudoacacia wood
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DOI：
10.1016/j.indcrop.2018.09.049
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发表时间：
2018-12-15
影响因子：
5.9
通讯作者：
Breton, Christian
中科院分区：
文献类型：
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作者：
Bostyn, Stephane;Destandau, Emilie;Breton, Christian
由于其快速生长和重要的生物量生产能力，刺槐（Robinia pseudoacacia L.）木材可能是一个有趣的天然化合物来源。在此，我们以80:20 （w:w）乙醇：水为溶剂，优化了该树种木材中积累的两种主要黄酮类化合物（即：robinetin和dihorobinetin）的提取工艺，该溶剂适合于进一步的工业用途，如化妆品。我们的实验设计主要关注两个主要提取参数：温度和木材/溶剂体积比，分别优化为27.5℃和177 g L-1。在这些条件下，提取液中二氢罗宾丁的最佳回收率约为3000 mg - L-1，罗宾丁的最佳回收率为700 mg - L-1。有趣的是，温度的影响可以忽略不计，从而减少工业水平的能源消耗。在实验的时间跨度内，知更鸟素和二氢知更鸟素浓度的演变分析揭示了这两种分子的相似行为。robintin和二氢robintin的释放动力学模型表明，准二级速率定律可以很好地描述提取过程，r(2)值大于0.91。最后，本研究的结果为扩大提取规模提供了有用的见解，并导致工业生产富含两种黄酮类化合物的刺槐木提取物。
Due to its rapid growth and important biomass production abilities, black locust (Robinia pseudoacacia L.) wood could represent an interesting source of natural compounds. Here, we optimized the extraction of the two main flavonoids accumulated in this species wood (i.e.: robinetin and dihydrorobinetin) with 80:20 (w:w) ethanol: water solvent well adapted for further industrial uses such as cosmetics. Our experimental design focused on two main extraction parameters: temperature and wood/solvent volume ratio that were optimized to 27.5 degrees C and 177 g L-1, respectively. These conditions lead to an optimal recovery of about 3000 mg L-1 of dihydrorobinetin and 700 mg L-1 of robinetin in the extracts. Interestingly, the effect of temperature could be neglected allowing reduced energy consumptions at the industrial level. Analysis of the evolution of robinetin and dihydrorobinetin concentrations during the timespan of the experiments revealed similar behaviours for both molecules. Kinetic modelling of robinetin and dihydrorobinetin release showed that pseudo-second order rate laws described well the extraction process with r(2) values over 0.91. In the end, the results of this study provided useful insights to scale-up the extractions and lead to an industrial production of black locust wood extracts enriched in both flavonoids of interest.