Transfecting mammalian cells: Optimization of critical parameters affecting calcium-phosphate precipitate formation
Transfecting mammalian cells: Optimization of critical parameters affecting calcium-phosphate precipitate formation
复制标题
DOI：
10.1093/nar/24.4.596
复制
发表时间：
1996-02-15
影响因子：
14.9
通讯作者：
Wurm, FM
中科院分区：
文献类型：
--
作者：
Jordan, M;Schallhorn, A;Wurm, FM
dna -磷酸钙共沉淀物在过饱和溶液中自发产生，然而，用于转染目的的高效沉淀物只能在非常狭窄的物理化学条件范围内产生，这些条件控制了沉淀复合物的产生和生长。钙和磷酸盐的浓度是影响沉淀复合物特性的主要因素，但其他参数，如温度，DNA浓度和反应时间也很重要，一个例子是发现几乎所有的可溶DNA在反应混合物中可以与磷酸钙结合成不溶的复合物
DNA-calcium phosphate co-precipitates arise spontaneously in supersaturated solutions, Highly effective precipitates for transfection purposes, however, can be generated only in a very narrow range of physico-chemical conditions that control the initiation and growth of precipitate complexes, The concentrations of calcium and phosphate are the main factors influencing characteristics of the precipitate complex, but other parameters, such as temperature, DNA concentration and reaction time are important as well, An example for this is the finding that almost all of the soluble DNA in the reaction mix can be bound into an insoluble complex with calcium phosphate in