The pigment characteristics and productivity shifting in high cell density culture of Monascus anka mycelia.
The pigment characteristics and productivity shifting in high cell density culture of Monascus anka mycelia.
复制标题
安卡红曲菌丝体高细胞密度培养中的色素特征和生产力转变
DOI：
10.1186/s12896-015-0183-3
复制
发表时间：
2015-08-13
影响因子：
3.5
通讯作者：
Wu Z
中科院分区：
文献类型：
--
作者：
Chen G;Shi K;Song D;Quan L;Wu Z
背景红曲菌丝体和色素具有潜在的药用价值和改善健康的功能，是有前景的食品和药物来源。使用红曲霉属的高细胞密度发酵。获得更高的菌丝体和黄色素产量值得研究。本研究对红曲霉 GIM 3.592 高细胞密度培养中色素的特性和生产力变化进行了研究。结果采用含有 C、N、P 和微量元素的补料培养基进行补料分批发酵，红曲菌丝体的高产量高达 39.77 g/L 细胞干重 (DCW)，是传统分批培养的四倍。但颜料总产量下降了 14.6%，这表明非耦合增长。补料培养后期，潜在的新型黄色颜料不断积累，导致颜料特性发生变化，黄色颜料成为主导颜料。橘霉素产量极低且与饲料成分无关。结论本研究提供了一种合适的发酵策略，可在高细胞密度培养物中生产具有高比例黄色素的功能性红曲菌丝体。首次报道了红曲高细胞密度培养物中色素生产力和特性变化。
BackgroundMonascus mycelia and pigments are promising sources of food and medicine with their potential pharmaceutical values and health-improving functions. Using high cell density fermentation of Monascus spp. to achieve higher mycelium and yellow pigment production is worthy to be researched. In this study, the characteristics and productivity shifting of pigments in high cell density culture of Monascus anka GIM 3.592 were investigated.ResultsThe high yield of Monascus mycelia up to 39.77 g/L dry cell weight (DCW), which was achieved by fed-batch fermentation with the feeding medium containing C, N, P and trace elements, was four times higher than that of conventional batch culture. But the total pigment production decreased by 14.6 %, which suggested non-coupled growth. Potential novel yellow pigments accumulated constantly at the late stage of the fed-batch culture, which resulted in a shift in pigment characteristics so that yellow pigments became the dominant pigments. Citrinin production was extremely low and independent of feeding ingredients.ConclusionsThis study provided a suitable fermentation strategy to produce functional Monascus mycelia with a high proportion of yellow pigments in high cell density culture. For the first time, it reported the pigment productivity and characteristics shifting in high cell density culture of Monascus.