FOURIER-ANALYSIS OF CELL MOTILITY - CORRELATION OF MOTILITY WITH METASTATIC POTENTIAL
FOURIER-ANALYSIS OF CELL MOTILITY - CORRELATION OF MOTILITY WITH METASTATIC POTENTIAL
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DOI：
10.1073/pnas.86.4.1254
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发表时间：
1989-02-01
影响因子：
11.1
通讯作者：
COFFEY, DS
中科院分区：
文献类型：
--
作者：
PARTIN, AW;SCHOENIGER, JS;COFFEY, DS
我们报告了用于定量各种类型细胞运动的计算机化数学系统的开发。这种傅里叶分析方法同时量化单个细胞（i）细胞形状的时间变化（以细胞皱褶、波动和伪足延伸为代表），（ii）细胞平移，以及（iii）平均细胞大小和形状。这种时空傅立叶分析在一系列明确表征的大鼠前列腺癌动物肿瘤细胞系上进行了测试，以定量方式研究细胞运动与体内转移潜力增加的相关性。测量伪足延伸的傅里叶运动系数与所研究的细胞系的转移潜力最相关。这项研究表明，傅里叶分析提供了细胞运动的定量测量，可应用于生物过程的研究。该分析应有助于研究细胞和肿瘤生物学各个领域中单个细胞的运动性。
We report the development of a computerized, mathematical system for quantitating the various types of cell motility. This Fourier analysis method simultaneously quantifies for individual cells (i) temporal changes in cell shape represented by cell ruffling, undulation, and pseudopodal extension, (ii) cell translation, and (iii) average cell size and shape. This spatial-temporal Fourier analysis was tested on a series of well-characterized animal tumor cell lines of rat prostatic cancer to study in a quantitative manner the correlation of cell motility with increasing in vivo metastatic potential. Fourier motility coefficients measuring pseudopodal extension correlated best with metastatic potential in the cell lines studied. This study demonstrated that Fourier analysis provides quantitative measurement of cell motility that may be applied to the study of biological processes. This analysis should aid in the study of the motility of individual cells in various areas of cellular and tumor biology.