Evaluation of the Effects of 810 nm Diode Laser Alone and in Combination With Gluma© and Chromophore on Dentinal Tubule Occlusion: A Scanning Electron Microscopic Analysis
Evaluation of the Effects of 810 nm Diode Laser Alone and in Combination With Gluma© and Chromophore on Dentinal Tubule Occlusion: A Scanning Electron Microscopic Analysis
复制标题
DOI：
10.34172/jlms.2020.45
复制
发表时间：
2020-06-01
影响因子：
1.5
通讯作者：
Moradi, Zohreh
中科院分区：
文献类型：
--
作者：
Khoubrouypak, Zahra;Tabatabaei, Masoumeh Hasani;Moradi, Zohreh
简介：牙齿过敏是最常见的患者投诉之一，由暴露的牙本质对外部刺激的反应引起。牙本质过敏症（DH）目前尚无确切的治疗方法。本研究的目的是比较单独的半导体激光器和Gluma和发色团在封闭开放的牙本质小管和治疗DH.Methods：这在体外研究进行了30个提取的人的第三磨牙保持在0.1%的百里酚溶液中的疗效。将牙齿固定在透明丙烯酸树脂中，并切割颊侧釉质以暴露牙本质的外三分之一。然后将样品分为6组阴性对照（未去除玷污层），阳性对照（涂抹层去除，使用6%柠檬酸），810 nm二极管激光照射（能量密度55.55J/cm（2），以连续模式1 W持续20秒），发色团（1 mg/mL吲哚菁绿色，ICG）加二极管激光照射（1 W，20秒）、Gluma加二极管激光照射和Gluma +发色团+二极管激光。在扫描电子显微镜下以2000倍放大率评价牙本质小管。牙本质小管阻塞的平均百分比报告为平均值和标准差。考虑到数据的正态分布，采用双因素方差分析比较治疗效果，两两比较采用独立样本t检验，
Introduction: Tooth hypersensitivity is among the most common patient complaints caused by the response of exposed dentin to external stimuli. No definite treatment has been suggested so far for dentin hypersensitivity (DH). This study aimed to compare the efficacy of the diode laser alone and in combination with Gluma and chromophore in occluding opened dentine tubules and the treatment of DH.Methods: This in vitro study was conducted on 30 extracted human third molars kept in 0.1% thymol solution. The teeth were mounted in transparent acrylic resin and the buccal enamel was cut to expose the outer third of dentin. The samples were then divided into 6 groups of negative control (no smear layer removal), positive control (smear layer removal with 6% citric acid application), 810 nm diode laser irradiation (energy density 55.55 J/cm(2), 1 W for 20 seconds in a continuous mode), chromophore (1 mg/mL indocyanine green, ICG) plus diode laser irradiation (1 W, 20 seconds), Gluma plus diode laser irradiation, and Gluma + chromophore + diode laser. Dentinal tubules were evaluated under a scanning electron microscope at x2000 magnification. The mean percentage of the obstruction of dentinal tubules was reported as mean and standard deviation. Considering the normal distribution of the data, two-way ANOVA was applied to compare the efficacy of treatments, and an independent-samples t test was used for pairwise comparisons at P